Updated and with approximately 25% new content, this textbook covers the latest developments, including instrumentation for microscopy and imaging, as well as current applications.The authors adopt a didactic approach, introducing infrared spectroscopy in a clear and well-structured way to provide students with a solid background in the principles and knowledge for efficiently using the method to obtain reliable results. Both beginners and experts will find up-to-date references for further reading.A must-have for advanced students (Master's and PhD) as well as those wanting to learn how the method works and how to work with it, including scientists from private and governmental labs.