Jens Kurreck, PhD, is a full Professor for Applied Biochemistry at the Technische Universität Berlin (TUB), Germany. He studied biochemistry and philosophy at the Free University of Berlin (FUB) and completed his PhD at the TUB in 1998. His research focuses on 3D bioprinting, RNA technologies, and virology. He has received several awards for his research achievements and teaching activities. Cy Aaron Stein, PhD, is Emeritus Professor of Oncology and Experimental Therapeutics and Chair of Medical Oncology at the City of Hope Medical Center in Duarte, CA, USA. He received a PhD in chemistry from Stanford University and an MD degree at the Albert Einstein College of Medicine, NY, in 1982. He has received numerous awards, among them the 2023 Life Achievement Award of the Oligonucleotide Therapeutics Society and a Clinical Career Development Award of the American Cancer Society.

Ein sehr gut strukturiertes, übersichtliches und sehr aktuelles Lehrbuch mit sehr gut erklärenden Abbildungen. (Dr. Ursula Hoja, Paracelsus Medizinische Universität, Nürnberg)

• Preface xiAcknowledgments xiiiAbout the Companion Website xv1 Introduction 11.1 The Basics of Molecular Medicine 21.2 The Human Cell 41.3 DNA Replication and Gene Expression 101.4 Biological Communication 261.5 The Immune System 312 Methods in Molecular Medicine 412.1 DNA Microarrays 422.2 Quantitative Polymerase Chain Reaction 452.3 Next-generation Sequencing 512.4 Proteomics 582.5 Animal Models in Biomedical Research 652.6 Alternatives to Animal Testing 682.7 Additional Methods 743 Genetic Disorders 813.1 Single-gene Disorders 823.2 Polygenic Disorders 1014 Molecular Oncology 1094.1 Basics of Oncology 1104.2 Selected Cancer Diseases 1124.3 Oncolytic Virus Therapy 1274.4 Cancer Stem Cells 1284.5 Cancer Immunotherapy 1305 Molecular Virology 1395.1 The Basics of Virology 1405.2 Vaccination 1495.3 Detection of Viruses 1595.4 Antiviral Therapy 1635.5 Prions 1726 Bacteria and Eukaryotic Pathogens 1776.1 Bacteria 1786.2 Eukaryotic Pathogens 1927 Genomics 1997.1 Whole Genome Sequencing 2007.2 The Human Genome 2027.3 Sequencing of Ancient DNA 2097.4 Follow-up Initiatives of the Human Genome Project 2118 Genetic Testing 2198.1 Types of Genetic Tests 2208.2 Chromosome Abnormalities 2248.3 Molecular Diagnosis 2299 Pharmacogenetics/Pharmacogenomics 2399.1 Uptake and Transport of Drugs 2429.2 Drug Metabolism 2429.3 Drug Targeting 2479.4 Drug Toxicity and Hypersensitivity 2509.5 Drug Development and Individual Pharmacotherapy 25010 Recombinant Protein Drugs 25510.1 Production of Recombinant Proteins 25810.2 Classes of Recombinant Drugs 26411 Gene Therapy 28711.1 Types of Gene Therapy 28811.2 Methods of Gene Transfer 28911.3 Tissue Specificity of Gene Transfer and Gene Expression 29711.4 Applications of Gene Therapy 30011.5 Genome Editing with CRISPR/Cas 30511.6 Future Prospects 30912 Stem Cells 31312.1 Embryonic Stem Cells 31512.2 Adult Stem Cells 31812.3 Induced Pluripotent Stem Cells 32012.4 Transdifferentiation and Direct Reprogramming 32312.5 Differentiation of Stem Cells 32512.6 Medical Applications of Stem Cells 32813 Oligonucleotide-based Strategies 34113.1 Antisense Oligonucleotides (ASOs) and Ribozymes 34313.2 RNA Interference 35113.3 MicroRNAs 36113.4 Aptamers, Decoy Oligonucleotides, and Immunostimulatory Oligonucleotides 36714 Ethics in Molecular Medicine 38314.1 The Basis of Bioethics 38414.2 Fields of Application 386Abbreviations 399Glossary 405Index 409

Jens Kurreck, PhD, is a full Professor for Applied Biochemistry at the Technische Universität Berlin (TUB), Germany. He studied biochemistry and philosophy at the Free University of Berlin (FUB) and completed his PhD at the TUB in 1998. His research focuses on 3D bioprinting, RNA technologies, and virology. He has received several awards for his research achievements and teaching activities. Cy Aaron Stein, PhD, is Emeritus Professor of Oncology and Experimental Therapeutics and Chair of Medical Oncology at the City of Hope Medical Center in Duarte, CA, USA. He received a PhD in chemistry from Stanford University and an MD degree at the Albert Einstein College of Medicine, NY, in 1982. He has received numerous awards, among them the 2023 Life Achievement Award of the Oligonucleotide Therapeutics Society and a Clinical Career Development Award of the American Cancer Society.

Ein sehr gut strukturiertes, übersichtliches und sehr aktuelles Lehrbuch mit sehr gut erklärenden Abbildungen. (Dr. Ursula Hoja, Paracelsus Medizinische Universität, Nürnberg)

• Preface xiAcknowledgments xiiiAbout the Companion Website xv1 Introduction 11.1 The Basics of Molecular Medicine 21.2 The Human Cell 41.3 DNA Replication and Gene Expression 101.4 Biological Communication 261.5 The Immune System 312 Methods in Molecular Medicine 412.1 DNA Microarrays 422.2 Quantitative Polymerase Chain Reaction 452.3 Next-generation Sequencing 512.4 Proteomics 582.5 Animal Models in Biomedical Research 652.6 Alternatives to Animal Testing 682.7 Additional Methods 743 Genetic Disorders 813.1 Single-gene Disorders 823.2 Polygenic Disorders 1014 Molecular Oncology 1094.1 Basics of Oncology 1104.2 Selected Cancer Diseases 1124.3 Oncolytic Virus Therapy 1274.4 Cancer Stem Cells 1284.5 Cancer Immunotherapy 1305 Molecular Virology 1395.1 The Basics of Virology 1405.2 Vaccination 1495.3 Detection of Viruses 1595.4 Antiviral Therapy 1635.5 Prions 1726 Bacteria and Eukaryotic Pathogens 1776.1 Bacteria 1786.2 Eukaryotic Pathogens 1927 Genomics 1997.1 Whole Genome Sequencing 2007.2 The Human Genome 2027.3 Sequencing of Ancient DNA 2097.4 Follow-up Initiatives of the Human Genome Project 2118 Genetic Testing 2198.1 Types of Genetic Tests 2208.2 Chromosome Abnormalities 2248.3 Molecular Diagnosis 2299 Pharmacogenetics/Pharmacogenomics 2399.1 Uptake and Transport of Drugs 2429.2 Drug Metabolism 2429.3 Drug Targeting 2479.4 Drug Toxicity and Hypersensitivity 2509.5 Drug Development and Individual Pharmacotherapy 25010 Recombinant Protein Drugs 25510.1 Production of Recombinant Proteins 25810.2 Classes of Recombinant Drugs 26411 Gene Therapy 28711.1 Types of Gene Therapy 28811.2 Methods of Gene Transfer 28911.3 Tissue Specificity of Gene Transfer and Gene Expression 29711.4 Applications of Gene Therapy 30011.5 Genome Editing with CRISPR/Cas 30511.6 Future Prospects 30912 Stem Cells 31312.1 Embryonic Stem Cells 31512.2 Adult Stem Cells 31812.3 Induced Pluripotent Stem Cells 32012.4 Transdifferentiation and Direct Reprogramming 32312.5 Differentiation of Stem Cells 32512.6 Medical Applications of Stem Cells 32813 Oligonucleotide-based Strategies 34113.1 Antisense Oligonucleotides (ASOs) and Ribozymes 34313.2 RNA Interference 35113.3 MicroRNAs 36113.4 Aptamers, Decoy Oligonucleotides, and Immunostimulatory Oligonucleotides 36714 Ethics in Molecular Medicine 38314.1 The Basis of Bioethics 38414.2 Fields of Application 386Abbreviations 399Glossary 405Index 409

Jens Kurreck, PhD, is a full Professor for Applied Biochemistry at the Technische Universität Berlin (TUB), Germany. He studied biochemistry and philosophy at the Free University of Berlin (FUB) and completed his PhD at the TUB in 1998. His research focuses on 3D bioprinting, RNA technologies, and virology. He has received several awards for his research achievements and teaching activities. Cy Aaron Stein, PhD, is Emeritus Professor of Oncology and Experimental Therapeutics and Chair of Medical Oncology at the City of Hope Medical Center in Duarte, CA, USA. He received a PhD in chemistry from Stanford University and an MD degree at the Albert Einstein College of Medicine, NY, in 1982. He has received numerous awards, among them the 2023 Life Achievement Award of the Oligonucleotide Therapeutics Society and a Clinical Career Development Award of the American Cancer Society.

Ein sehr gut strukturiertes, übersichtliches und sehr aktuelles Lehrbuch mit sehr gut erklärenden Abbildungen. (Dr. Ursula Hoja, Paracelsus Medizinische Universität, Nürnberg)

• Preface xiAcknowledgments xiiiAbout the Companion Website xv1 Introduction 11.1 The Basics of Molecular Medicine 21.2 The Human Cell 41.3 DNA Replication and Gene Expression 101.4 Biological Communication 261.5 The Immune System 312 Methods in Molecular Medicine 412.1 DNA Microarrays 422.2 Quantitative Polymerase Chain Reaction 452.3 Next-generation Sequencing 512.4 Proteomics 582.5 Animal Models in Biomedical Research 652.6 Alternatives to Animal Testing 682.7 Additional Methods 743 Genetic Disorders 813.1 Single-gene Disorders 823.2 Polygenic Disorders 1014 Molecular Oncology 1094.1 Basics of Oncology 1104.2 Selected Cancer Diseases 1124.3 Oncolytic Virus Therapy 1274.4 Cancer Stem Cells 1284.5 Cancer Immunotherapy 1305 Molecular Virology 1395.1 The Basics of Virology 1405.2 Vaccination 1495.3 Detection of Viruses 1595.4 Antiviral Therapy 1635.5 Prions 1726 Bacteria and Eukaryotic Pathogens 1776.1 Bacteria 1786.2 Eukaryotic Pathogens 1927 Genomics 1997.1 Whole Genome Sequencing 2007.2 The Human Genome 2027.3 Sequencing of Ancient DNA 2097.4 Follow-up Initiatives of the Human Genome Project 2118 Genetic Testing 2198.1 Types of Genetic Tests 2208.2 Chromosome Abnormalities 2248.3 Molecular Diagnosis 2299 Pharmacogenetics/Pharmacogenomics 2399.1 Uptake and Transport of Drugs 2429.2 Drug Metabolism 2429.3 Drug Targeting 2479.4 Drug Toxicity and Hypersensitivity 2509.5 Drug Development and Individual Pharmacotherapy 25010 Recombinant Protein Drugs 25510.1 Production of Recombinant Proteins 25810.2 Classes of Recombinant Drugs 26411 Gene Therapy 28711.1 Types of Gene Therapy 28811.2 Methods of Gene Transfer 28911.3 Tissue Specificity of Gene Transfer and Gene Expression 29711.4 Applications of Gene Therapy 30011.5 Genome Editing with CRISPR/Cas 30511.6 Future Prospects 30912 Stem Cells 31312.1 Embryonic Stem Cells 31512.2 Adult Stem Cells 31812.3 Induced Pluripotent Stem Cells 32012.4 Transdifferentiation and Direct Reprogramming 32312.5 Differentiation of Stem Cells 32512.6 Medical Applications of Stem Cells 32813 Oligonucleotide-based Strategies 34113.1 Antisense Oligonucleotides (ASOs) and Ribozymes 34313.2 RNA Interference 35113.3 MicroRNAs 36113.4 Aptamers, Decoy Oligonucleotides, and Immunostimulatory Oligonucleotides 36714 Ethics in Molecular Medicine 38314.1 The Basis of Bioethics 38414.2 Fields of Application 386Abbreviations 399Glossary 405Index 409