Optical Fluorescence Microscopy

From the reviews: "This book offers a recent approach to applications of microscopy for the examination of biological processes and brings you to an introduction to the complex world of optical microscopy. ... should be available in all schools, research laboratories, community and university libraries. A good book for the scientists working in molecular and cell biology, biochemistry, medicine, pharmacology and biophysics." (Samantha C. Karunarathna, Fungal Diversity, April, 2012)

• Fundamentals of Optical Microscopy.- The White Confocal - Continuous Spectral Tuning in Excitation and Emission.- SHG/THG (Second/Third Harmonic Generation) Microscopy.- Role of Scattering and Nonlinear Effects in the Illumination and the Photobleaching Distribution Profiles.- New Analytical Tools for Evaluation of Spherical Aberration in Optical Microscopy.- Improving Image Formation by Pushing the Signal to Noise Ratio.- Site-specific Labeling of Proteins in Living Cells Using Synthetic Fluorescent Dyes.- Imaging Molecular Physiology in Cells Using FRET-based Fluorescent Nanosensors.- Measuring Molecular Dynamics by FRAP, FCS and SPT.- In vitro – in-vivo Fluctuation Spectroscopies.- Interference X-ray Diffraction from Single Muscle Cells Reveals the Molecular Basis of Muscle Braking.- Low Concentration Protein Detection Using Novel SERS Devices.- Near Infrared 3-Dimensional Nonlinear Optical Monitoring of Stem Cell Differentiation.- A Correlative Microscopy: a Combination of Light and Electron Microscopy.