This book constitutes the refereed proceedings of the 2nd International Symposium on Software Fault Prevention, Verification, and Validation, SFPVV 2025, held in Shanghai, China, during November 8–9, 2025.The 11 full papers included in this book were carefully reviewed and selected from 31 submissions. They are grouped into the following topics: AI-driven approaches, and their combinations can be explored, established.