Amit Shukla – författare

Experimental and Evidence based Protocols in Pharmacology provides a holistic coverage of scientific protocols involved in the pharmacology and toxicological studies. The book serves as a reference to the biomedical researchers working in pharmacology and allied subjects to mitigate the challenges in the routine laboratory procedures. Book has 22 chapters with evidence based protocols and procedures to conduct the defined experiment. Further, this book provides single reference window with evidence based practical approaches to cater the need of academician and research scholars. The book is designed in a diligent manner to provide majority of protocols viz. Primary cell culture, Mesenchymal stem cells isolation and its application, DNA fragmentation Assay, oxidative stress evaluation protocols, Cytotoxicity and Genotoxicity Assays, Apoptosis studies, Analytical studies involving HPLC, GC-MS, ICP-OES and experimental pharmacological tool in smooth muscle pharmacological studies etc. to cement the scientific acumen. This book imbibes all the basic and advanced research protocols along with their translational approaches in experiments involved in pharmacology and also provides the insight of example based drug assays to help in bioprospecting of naive molecules.Further, this book also provide a reference book to the researchers involved in toxicological testing using OECD testing guideline to calculate the lethal dose of any compound in a better manner. This MS also suffice the different methods to calculate the median lethal dose (LD50). The important reproductive toxicity assays to ascertain the toxic potential of any xenobiotics is also incorporated in this MS. Moreover, sophisticated technique like Flow cytometry and other experimental models viz. Direct and Indirect LPS induced acute lung injury model has also well written to provide an insight of the experimentation involving laboratory animals.

Toxicology is a fundamental subject in both Veterinary and Medical sciences. It is a vast field with numerous branches and has been studied extensively since ancient times. It is essential to possess a strong foundation in the basic concepts of general toxicology, as well as the modes of toxicity of different plants and mycotoxins. The sources of poisons also include natural sources like plant and fungal toxins. This book provides a comprehensive overview of toxicodynamics, diagnosis, and treatment of several plant toxins and mycotoxins. To facilitate easy understanding, the book has been written in a notes format and in simple language. Important points from an exam perspective have been highlighted in bold letters, and the book also includes pictorial depictions of plant plates.The book comprises of 15 chapters that cover a range of topics related to general toxicology, including its history, sources, factors affecting toxicity, toxicokinetics, and basic principles of treatment for poisoning and toxicology of different plants and mycotoxins.

Pharmacology and Toxicology is a subject that imposes a great responsibility on the veterinary and medical practitioner in their day to day clinical practice. Conceptualizing the concepts and making them at ease to the budding vets is the need of the hour. The central dogma of revisiting the concepts lies well with the practices of the objective type questions to help individuals in attaining a great success in their academic and professional pursuit. This book contains 11 chapters encompassing the multiple-choice questions, true false and fill in the blanks vis a vis matching to consolidate the scientific acumen of the students in brightening their future. The main highlight of this book is the vast coverage of new chapters related to drug discovery, multi drug resistance (MDR) and clinical pharmacology and Veterinary therapeutics.