Arthur Germano Fett-Neto – författare

This second edition provides detailed practical information on important methods employed in the engineering of plant secondary metabolism pathways. New and updated chapters guide readers through extraction, quantification, purification, localization, characterization, data mining and processing, biosynthesis modulation, and pathway engineering of representative classes of plant specialized metabolites. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, list of necessary materials and reagents, tips on troubleshooting and known pitfalls, and step-by-step descriptions of readily reproducible protocols.

Authoritative and cutting-edge, Plant Secondary Metabolism Engineering: Methods and Protocols, Second Edition aims to be a useful practical guide to help researchers working in this exciting field. 

This volume describes up-to-date techniques for improved production of secondary metabolites of economic interest using field and laboratory methods. Biotechnology of Plant Secondary Metabolism: Methods and Protocols explores different secondary metabolite classes, whole-plant and cell/organ culture systems, and environmental and genetic transformation-based modulation of biochemical pathways. Special focus is given to cell and tissue specific metabolism, metabolite transport, microRNA-based technology, heterologous systems expression of enzymes and pathways leading to products of interest, as well as applications using both model and non-model plant species. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Practical and cutting-edge, Biotechnology of Plant Secondary Metabolism: Methods and Protocols is a great resource for scientists of interdisciplinary fields--plant science, plant physiology, pharmacy, molecular biology, biochemistry, bioengineering, and forestry--in reaching their goals of producing plant biochemicals in a sustainable and efficient manner, while minimizing impacts to the environment and providing the required quantities of these commodities to industry.