Constantinos M. Mikelis – författare

This volume provides an overview of advanced protocols for vascular biology research, comprising cardiovascular and lymphatic biology. Chapters detail techniques for isolating and characterizing endothelial and mural cell populations, disseminating signaling pathways and protein-protein interactions, constructing organ-on-chip systems, and using animal models and techniques with bench-to-bedside potential. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Methods in Cardiovascular and Lymphatic Biology aims to provide protocols and applications that can be used by both new investigators and experienced professionals to advance cardiovascular and lymphatic research.

In the vasculature, the extracellular matrix (ECM) is involved in the regulation of angiogenesis, vascular mechanosensing, and blood vessel stability.This book aims to provide the reader with an overview of the various roles of the ECM during angiogenesis and covers its important role for the maintenance of vascular integrity, capillary and arterial morphogenesis, as well as lymphangiogenesis. Furthermore, aspects of regulation of endothelial cell and pericyte functions by the ECM under physiological and pathological conditions are discussed. In addition, the reader will learn more about different approaches to exploit ECM molecules for designing therapeutic approaches or as biomarkers to improve therapeutic decisions.Comprehensive and cutting-edge, Matrix Pathobiology and Angiogenesis is a valuable resource for experienced researchers and early career scientist alike, who are interested in in learning more about this exciting and developing field.The series Biology of Extracellular Matrix is published in collaboration with the American Society for Matrix Biology and the International Society for Matrix Biology.