David R. O'Reilly - Böcker

This laboratory manual simplifies selection of the most appropriate baculovirus vector design for a given problem. It then describes each step of the implementation process - from vector construction to large-scale protein production. The book provides an understanding of how the vectors work, a biological overview of cells, viruses, plasmids, and promoters, guidelines for choosing optimum vectors, protocols for growing insect cells and recombinant viruses, methods for analysing protein products and scaling up protein production, techniques for producing proteins in insect larvae, and easy to use maps charting available expression vectors.