Bacterial Laccases: Engineering, Immobilization, Heterologous Production, and Industrial Applications provides a list of approaches that upgrades bacterial laccases to industrially relevant enzymes. Providing protocols for enzyme production and downstream processing, and including up-to-date information on bacterial laccases, as well as techniques that can be explored to enhance properties and production levels, this book clarifies research gaps and explains how they can be dealt with. Written for research scholars working on enzymes, this is a valuable tool for industries and policymakers.- Leads to a better understanding of traditional and novel methodologies for enhancing production and properties of bacterial laccases- Serves as a useful guide for researchers, industrialists and students- Includes chapters written by experts known for their contributions in respective areas- Describes the latest advances made in the field of bacterial laccases, including strategies that could be undertaken to improve their utility at an industrial level- Provides a comprehensive, up-to-date review of bacterial laccases and their important applications

Bacterial Laccases: Engineering, Immobilization, Heterologous Production, and Industrial Applications provides a list of approaches that upgrades bacterial laccases to industrially relevant enzymes. Providing protocols for enzyme production and downstream processing, and including up-to-date information on bacterial laccases, as well as techniques that can be explored to enhance properties and production levels, this book clarifies research gaps and explains how they can be dealt with. Written for research scholars working on enzymes, this is a valuable tool for industries and policymakers. Leads to a better understanding of traditional and novel methodologies for enhancing production and properties of bacterial laccases Serves as a useful guide for researchers, industrialists and students Includes chapters written by experts known for their contributions in respective areas Describes the latest advances made in the field of bacterial laccases, including strategies that could be undertaken to improve their utility at an industrial level Provides a comprehensive, up-to-date review of bacterial laccases and their important applications

Fundamentals of Recombinant Protein Production, Purification and Characterization is organized into nine chapters in a logical fashion that cover an introduction to recombinant proteins and expression in different host expression systems, extraction, purification and analysis of proteins. This important reference features protocols, along with the advantages and disadvantage of each expression hosts and characterization technique (presented in tabular format) and offers detailed coverage of all aspects of protein production and processing (upstream and downstream processing) in one place. Finally, the book ends with different characterization techniques. Production of recombinant proteins for biotechnological and therapeutic applications at a large scale is an essential need of mankind. With the huge application potential of therapeutic and industrial proteins, there has been increasing demand for effective and efficient bioprocessing strategies. Recent progress around recombinant DNA technologies and bioprocessing strategies has paved the way for efficient production of recombinant proteins. Important factors such as insolubility and cost of production need to be considered for large scale production of these recombinant proteins.

Includes step-by-step reproducible protocols while also providing updated information on the rationale and latest developments in expression systemsCan also be used as a handbook for protein expression and purification as expression systems and chromatographic methods are explained in detailConsists of notes on troubleshooting from the eminent researchers in the fieldProvides comprehensive information on protein production, purification and characterization in a single volumeDescribes different purification methods for comparatively difficult to obtain proteinsBrings the topics of recombinant protein expression, purification and characterization together, thereby making it the first resource on how to solve problems with respect to upstream and downstream processing of heterologous proteins

Fundamentals of Recombinant Protein Production, Purification and Characterization is organized into nine chapters in a logical fashion that cover an introduction to recombinant proteins and expression in different host expression systems, extraction, purification and analysis of proteins. This important reference features protocols, along with the advantages and disadvantage of each expression hosts and characterization technique (presented in tabular format) and offers detailed coverage of all aspects of protein production and processing (upstream and downstream processing) in one place. Finally, the book ends with different characterization techniques.Production of recombinant proteins for biotechnological and therapeutic applications at a large scale is an essential need of mankind. With the huge application potential of therapeutic and industrial proteins, there has been increasing demand for effective and efficient bioprocessing strategies. Recent progress around recombinant DNA technologies and bioprocessing strategies has paved the way for efficient production of recombinant proteins. Important factors such as insolubility and cost of production need to be considered for large scale production of these recombinant proteins.- Includes step-by-step reproducible protocols while also providing updated information on the rationale and latest developments in expression systems- Can also be used as a handbook for protein expression and purification as expression systems and chromatographic methods are explained in detail- Consists of notes on troubleshooting from the eminent researchers in the field- Provides comprehensive information on protein production, purification and characterization in a single volume- Describes different purification methods for comparatively difficult to obtain proteins- Brings the topics of recombinant protein expression, purification and characterization together, thereby making it the first resource on how to solve problems with respect to upstream and downstream processing of heterologous proteins

Bacterial Laccases: Engineering, Immobilization, Heterologous Production, and Industrial Applications provides a list of approaches that upgrades bacterial laccases to industrially relevant enzymes. Providing protocols for enzyme production and downstream processing, and including up-to-date information on bacterial laccases, as well as techniques that can be explored to enhance properties and production levels, this book clarifies research gaps and explains how they can be dealt with. Written for research scholars working on enzymes, this is a valuable tool for industries and policymakers.- Leads to a better understanding of traditional and novel methodologies for enhancing production and properties of bacterial laccases- Serves as a useful guide for researchers, industrialists and students- Includes chapters written by experts known for their contributions in respective areas- Describes the latest advances made in the field of bacterial laccases, including strategies that could be undertaken to improve their utility at an industrial level- Provides a comprehensive, up-to-date review of bacterial laccases and their important applications

Bacterial Laccases: Engineering, Immobilization, Heterologous Production, and Industrial Applications provides a list of approaches that upgrades bacterial laccases to industrially relevant enzymes. Providing protocols for enzyme production and downstream processing, and including up-to-date information on bacterial laccases, as well as techniques that can be explored to enhance properties and production levels, this book clarifies research gaps and explains how they can be dealt with. Written for research scholars working on enzymes, this is a valuable tool for industries and policymakers. Leads to a better understanding of traditional and novel methodologies for enhancing production and properties of bacterial laccases Serves as a useful guide for researchers, industrialists and students Includes chapters written by experts known for their contributions in respective areas Describes the latest advances made in the field of bacterial laccases, including strategies that could be undertaken to improve their utility at an industrial level Provides a comprehensive, up-to-date review of bacterial laccases and their important applications

Fundamentals of Recombinant Protein Production, Purification and Characterization is organized into nine chapters in a logical fashion that cover an introduction to recombinant proteins and expression in different host expression systems, extraction, purification and analysis of proteins. This important reference features protocols, along with the advantages and disadvantage of each expression hosts and characterization technique (presented in tabular format) and offers detailed coverage of all aspects of protein production and processing (upstream and downstream processing) in one place. Finally, the book ends with different characterization techniques. Production of recombinant proteins for biotechnological and therapeutic applications at a large scale is an essential need of mankind. With the huge application potential of therapeutic and industrial proteins, there has been increasing demand for effective and efficient bioprocessing strategies. Recent progress around recombinant DNA technologies and bioprocessing strategies has paved the way for efficient production of recombinant proteins. Important factors such as insolubility and cost of production need to be considered for large scale production of these recombinant proteins.

Includes step-by-step reproducible protocols while also providing updated information on the rationale and latest developments in expression systemsCan also be used as a handbook for protein expression and purification as expression systems and chromatographic methods are explained in detailConsists of notes on troubleshooting from the eminent researchers in the fieldProvides comprehensive information on protein production, purification and characterization in a single volumeDescribes different purification methods for comparatively difficult to obtain proteinsBrings the topics of recombinant protein expression, purification and characterization together, thereby making it the first resource on how to solve problems with respect to upstream and downstream processing of heterologous proteins

Fundamentals of Recombinant Protein Production, Purification and Characterization is organized into nine chapters in a logical fashion that cover an introduction to recombinant proteins and expression in different host expression systems, extraction, purification and analysis of proteins. This important reference features protocols, along with the advantages and disadvantage of each expression hosts and characterization technique (presented in tabular format) and offers detailed coverage of all aspects of protein production and processing (upstream and downstream processing) in one place. Finally, the book ends with different characterization techniques.Production of recombinant proteins for biotechnological and therapeutic applications at a large scale is an essential need of mankind. With the huge application potential of therapeutic and industrial proteins, there has been increasing demand for effective and efficient bioprocessing strategies. Recent progress around recombinant DNA technologies and bioprocessing strategies has paved the way for efficient production of recombinant proteins. Important factors such as insolubility and cost of production need to be considered for large scale production of these recombinant proteins.- Includes step-by-step reproducible protocols while also providing updated information on the rationale and latest developments in expression systems- Can also be used as a handbook for protein expression and purification as expression systems and chromatographic methods are explained in detail- Consists of notes on troubleshooting from the eminent researchers in the field- Provides comprehensive information on protein production, purification and characterization in a single volume- Describes different purification methods for comparatively difficult to obtain proteins- Brings the topics of recombinant protein expression, purification and characterization together, thereby making it the first resource on how to solve problems with respect to upstream and downstream processing of heterologous proteins