Donald C. Chang – författare

Electroporation is an efficient method to introduce macromolecules such as DNA into a wide variety of cells. Electrofusion results in the fusion of cells and can be used to produce genetic hybrids or hybridoma cells.Guide to Electroporation and Electrofusion is designed to serve the needs of students, experienced researchers, and newcomers to the field. It is a comprehensive manual that presents, in one source, up-to-date, easy-to-follow protocols necessary for efficient electroporation and electrofusion of bacteria, yeast, and plant and animal cells, as well as background information to help users optimize their results through comprehension of the principles behind these techniques.- Covers fundamentals of electroporation and electrofusion in detail: Molecular events, Mechanisms, Kinetics, Gives extensive practical information, The latest applications, Controlling parameters to maximize efficiency, Available instrumentation- Presents applications of electroporation and electrofusion in current research situations- State-of-the-art modifications to electrical pulses and generators- Application of electroporation and electrofusion to unique, alternative cell and tissue types- Gives straightforward, detailed, easy-to-follow protocols for Formation of human hybridomas- Introduction of genetic material into plant cells and pollen- Transfection of mammalian cells- Transformation of bacteria, plants, and yeast- Production of altered embryos- Optimization of electroporation by using reporter genes- Comprehensive and up-to-date- Convenient bench-top format- Approximately 125 illustrations complement the text- Complete references with article titles- Written by leading authorities in electroporation and electrofusion

This book presents a new approach to understanding the foundation of quantum physics through the "quantum wave model" hypothesis. It addresses some of the key challenges in the current quantum theory, including the conflict between quantum mechanics and relativity, and offers a comprehensive solution to many of the existing mysteries in the field. By proposing that the vacuum is a dielectric medium and quantum particles are quantized excitation waves of the vacuum, the book provides a clear physical interpretation of wave-particle duality and explains the physical basis of energy, momentum, and mass.With topics ranging from the physical foundation of quantum mechanics to the derivation of the quantum wave equations and the resolution of the conflict between quantum physics and relativity, this book offers a comprehensive overview of the most pressing issues in the field. Written at a level accessible to undergraduate students and senior researcher scientists alike,this book offers a valuable resource for anyone seeking a deeper understanding of quantum mechanics and its fundamental role in shaping our understanding of the physical world.