Francisco J. Corpas - Böcker

Oxygen, Nitrogen and Sulfur Species in Post-harvest Physiology of Horticultural Crops, a volume in the Plant Gasotransmitter series, analyzes the latest advances in post-harvest physiology. The book presents metabolic cascades and highlights the role of gasotransmitters as intercellular regulators of metabolic processes. Post-harvest physiology differs between climacteric and non-climacteric fruits and vegetables, as well as for fresh-cut flowers and non-food plants. Initial chapters review the cascades, intercellular pathways and messenger molecules that drive ripeness and longevity, presenting the chemistry behind key pathways. The books also takes a deep dive into core gasotransmitters, describing the data behind known properties, chemistry and physiological roles.

Applications for prolonging shelf-life via the control of post-harvest fungi, bacteria and omics approaches are reviewed in detail, offering readers guidance on how to put gasotransmitters research into practice. This is an essential resource for students, researchers and agronomists interested in plant physiology, biochemistry and plant hormones.

Describes the use and application of oxygen, nitrogen and sulfur species towards the prolonging of post-harvest shelf-life in agricultural products Explores eco-friendly alternatives to hazardous chemical compounds used to preserve fruits Presents metabolic cascades and evaluates the crosstalk and interaction of gasotransmitters within these cascades

This detailed volume presents methodologies for studying reactive oxygen species (ROS) metabolism in plant cells, including techniques for detecting different types of ROS such as superoxide radicals, hydrogen peroxide or singlet oxygen, as well as ROS generating systems such as xanthine oxidoreductase. The book provides alternatives to determine malondialdehyde, electrochemical detection approaches for determining total antioxidant capacity (TAC) as well as enzymatic antioxidants, as well as methods for studying non-enzymatic antioxidants and some components of the secondary metabolism. Written for the highly successful Methods in Molecular Biology series, the chapters in this book provide the kind of detailed implementation advice that leads to ideal results in the laboratory.  Authoritative and practical, ROS Signaling in Plants: Methods and Protocols serves as an ideal guide for scientists interested in the metabolism of ROS in plant cells. Chapter “Noninvasive In Planta Live Measurements of H2O2 and Glutathione Redox Potential with Fluorescent roGFPs-Based Sensors” is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.

This detailed volume presents methodologies for studying reactive oxygen species (ROS) metabolism in plant cells, including techniques for detecting different types of ROS such as superoxide radicals, hydrogen peroxide or singlet oxygen, as well as ROS generating systems such as xanthine oxidoreductase.

This book provides an overview of antioxidants and antioxidant enzymes and their role in the mechanisms of signaling and cellular tolerance under stress in plant systems. Major reactive oxygen species (ROS)-scavenging/modulating enzymes include the superoxide dismutase (SOD) that dismutates O2 into H2O2, which is followed by the coordinated action of a set of enzymes including catalase (CAT), ascorbate peroxidase (APX), glutathione peroxidase (GPX) and peroxiredoxins (Prx) that remove H2O2. In addition to the ROS scavenging enzymes, a number of other enzymes are found in various subcellular compartments, which are involved in maintaining such redox homeostasis either by directly scavenging particular ROS and ROS-byproducts or by replenishing antioxidants. In that respect, these enzymes can be also considered antioxidants. Such enzymes include monodehydroascorbate reductase (MDAR), dehydroascorbate reductase (DHAR), glutathione reductase (GR), alternative oxidases (AOXs), peroxidases (PODs) and glutathione S-transferases (GSTs). Some non-enzymatic antioxidants, such as ascorbic acid (vitamin C), carotenes (provitamin A), tocopherols (vitamin E), and glutathione (GSH), work in concert with antioxidant enzymes to sustain an intracellular steady-state level of ROS that promotes plant growth, development, cell cycles and hormone signaling, and reinforces the responses to abiotic and biotic environmental stressors. Offering a unique compilation of information on antioxidants and antioxidant enzymes, this is a valuable resource for advanced students and researchers working on plant biochemistry, physiology, biotechnology, and signaling in cell organelles, and those specializing in plant enzyme technology.

This book describes nitric oxide (NO) and hydrogen peroxide (H2O2) functions in higher plants. Much progress has been made in the field of NO and H2O2 research regarding the various mechanisms and functions of these two molecules, particularly regarding stress tolerance and signaling processes, but there are still gaps to be filled. NO and H2O2 are both crucial regulators of development, and act as signaling molecules at each step of the plant lifecycle, while also playing important roles in biotic and abiotic responses to environmental cues. The book summarizes key advances in the field of NO and H2O2 research, focusing on a range of processes including: signaling, metabolism, seed germination, development, sexual reproduction, fruit ripening, and defense.

This book focuses on the role of hydrogen sulfide in the protection of plants against abiotic stresses and abiotic stress-induced complications by the way of converging advanced key methods of proteomics, genomics, and metabolomics.

This book focuses on the role of hydrogen sulfide in the protection of plants against abiotic stresses and abiotic stress-induced complications by the way of converging advanced key methods of proteomics, genomics, and metabolomics.

This book provides an overview of the recent advancements for plant scientists with a research focus on phytohormones and their responses (nature, occurrence, and functions) in plant cells. This book focuses on the role of phytohormones in biosynthesis, plant sexual reproduction, seed germination and fruit development and ripening.

This book provides an overview of the recent advancements for plant scientists with a research focus on phytohormones and their responses (nature, occurrence, and functions) in plant cells. This book focuses on the role of phytohormones in biosynthesis, plant sexual reproduction, seed germination and fruit development and ripening.

The new edited volume on phytomelatonin and its diverse roles in plants under a challenging environment shall be an important reference book with updated information and future perspectives on the involvement of this biomolecule in stress resilience in plants.

The new edited volume on phytomelatonin and its diverse roles in plants under a challenging environment shall be an important reference book with updated information and future perspectives on the involvement of this biomolecule in stress resilience in plants.

Given the multidimensional role of these signaling molecules, research over the past decades in mitigating abiotic stresses in plant biology and from an agriculture point of view, we intend to bring forth a comprehensive volume “Gasotransmitters Signaling in Plants under Challenging Environment".

Given the multidimensional role of these signaling molecules, research over the past decades in mitigating abiotic stresses in plant biology and from an agriculture point of view, we intend to bring forth a comprehensive volume “Gasotransmitters Signaling in Plants under Challenging Environment".

This book covers the key features of nitric oxide (NO) in plants. and on its signaling and interaction with phytohormones, mineral nutrients, biomolecules, ions and ion channels in plants under abiotic stresses.

This book offers an up-to-date review of the regulatory role of nitric oxide (NO) changes in the morphological, physio-biochemical as well as molecular characteristics of plants under abiotic stress.

This book provides detailed and comprehensive information on oxidative damage caused by stresses in plants with especial reference to the metabolism of reactive oxygen species (ROS).In plants, as in all aerobic organisms, ROS are common by-products formed by the inevitable leakage of electrons onto O2 from the electron transport activities located in chloroplasts, mitochondria, peroxisomes and in plasma membranes or as a consequence of various metabolic pathways confined in different cellular loci. Environmental stresses such as heat, cold, drought, salinity, heavy-metal toxicity, ozone and ultraviolet radiation as well as pathogens/contagion attack lead to enhanced generation of ROS in plants due to disruption of cellular homeostasis. ROS play a dual role in plants; at low concentrations they act as signaling molecules that facilitate several responses in plant cells, including those promoted by biotic and abiotic agents. In divergence, at high levels they cause damage to cellular constituents triggering oxidative stress. In either case, small antioxidant molecules and enzymes modulate the action of these ambivalent species.

This book offers an up-to-date review of the regulatory role of nitric oxide (NO) changes in the morphological, physio-biochemical as well as molecular characteristics of plants under abiotic stress.

This book provides detailed and comprehensive information on oxidative damage caused by stresses in plants with especial reference to the metabolism of reactive oxygen species (ROS).In plants, as in all aerobic organisms, ROS are common by-products formed by the inevitable leakage of electrons onto O2 from the electron transport activities located in chloroplasts, mitochondria, peroxisomes and in plasma membranes or as a consequence of various metabolic pathways confined in different cellular loci. Environmental stresses such as heat, cold, drought, salinity, heavy-metal toxicity, ozone and ultraviolet radiation as well as pathogens/contagion attack lead to enhanced generation of ROS in plants due to disruption of cellular homeostasis. ROS play a dual role in plants; at low concentrations they act as signaling molecules that facilitate several responses in plant cells, including those promoted by biotic and abiotic agents. In divergence, at high levels they cause damage to cellular constituents triggering oxidative stress. In either case, small antioxidant molecules and enzymes modulate the action of these ambivalent species.

This book covers the key features of nitric oxide (NO) in plants. and on its signaling and interaction with phytohormones, mineral nutrients, biomolecules, ions and ion channels in plants under abiotic stresses.

This book provides an up-to-date overview of redox signaling in plant cells and its key role in responses to different stresses. cellular redox homeostasis as a central modulator; The book is an invaluable source of information for plant scientists and students interested in redox state chemistry and cellular tolerance in plants.

This book provides an overview of antioxidants and antioxidant enzymes and their role in the mechanisms of signaling and cellular tolerance under stress in plant systems. Major reactive oxygen species (ROS)-scavenging/modulating enzymes include the superoxide dismutase (SOD) that dismutates O2 into H2O2, which is followed by the coordinated action of a set of enzymes including catalase (CAT), ascorbate peroxidase (APX), glutathione peroxidase (GPX) and peroxiredoxins (Prx) that remove H2O2. In addition to the ROS scavenging enzymes, a number of other enzymes are found in various subcellular compartments, which are involved in maintaining such redox homeostasis either by directly scavenging particular ROS and ROS-byproducts or by replenishing antioxidants. In that respect, these enzymes can be also considered antioxidants. Such enzymes include monodehydroascorbate reductase (MDAR), dehydroascorbate reductase (DHAR), glutathione reductase (GR), alternative oxidases (AOXs), peroxidases (PODs) and glutathione S-transferases (GSTs). Some non-enzymatic antioxidants, such as ascorbic acid (vitamin C), carotenes (provitamin A), tocopherols (vitamin E), and glutathione (GSH), work in concert with antioxidant enzymes to sustain an intracellular steady-state level of ROS that promotes plant growth, development, cell cycles and hormone signaling, and reinforces the responses to abiotic and biotic environmental stressors. Offering a unique compilation of information on antioxidants and antioxidant enzymes, this is a valuable resource for advanced students and researchers working on plant biochemistry, physiology, biotechnology, and signaling in cell organelles, and those specializing in plant enzyme technology.

This book provides an up-to-date overview of redox signaling in plant cells and its key role in responses to different stresses. cellular redox homeostasis as a central modulator; The book is an invaluable source of information for plant scientists and students interested in redox state chemistry and cellular tolerance in plants.

Plants possess a range of potential cellular mechanisms that may be involved in the detoxification of heavy metals and thus tolerance to metal stress.