J. Paul Robinson - Böcker

Cytometry is characterization and measurement of cells and cellular constituents, most often used to immunophenotype cells - that is, to distinguish healthy cells from diseased cells. Flow Cytometry specifically is quite sensitive, allowing researchers to detect rare cell types and residual levels of disease, and as such has been the method of choice for important studies such as monitoring the blood of AIDS patients. For this reason, there is a great need for a practical, comprehensive manual that will be useful across a broad range of laboratories. This volume, as part of the Reliable Lab Solution Series, delivers such a tool, offering busy researchers across many disciplines a handy resource of all the best methods and protocols for Cytometry to use at the bench. Highlights top downloaded and cited chapters, authored by pioneers in the field and enhanced with their tips, and pitfalls to avoid Loaded with detailed protocols developed and used by leaders in the field Refines, organizes and updates popular methods from one of our top selling series, Methods in Cell Biology

Advances in Cytometry: Applications, Volume 176 in the Methods in Cell Biology series, highlights advances in the field, with this new volume presenting interesting chapters on timely topics, including Orthotopic brain tumor models derived from glioblastoma stem-like cells, RNA sequencing in hematopoietic stem cells, Generation of inducible pluripotent stem cells from human dermal fibroblasts, In vitro preparation of dental pulp stem cell grafts combined with biocompatible scaffolds for tissue engineering, Gene expression knockdown in chronic myeloid leukemia stem cells, Identification and isolation of slow-cycling GSCs, Assessment of CD133, EpCAM, and much more. Provides the authority and expertise of leading contributors from an international board of authors Presents the latest release in the Methods in Cell Biology series Includes the latest information on the topic of development, characterization and applications in CAR T Cells

Handbook of Flow Cytometry Methods Edited By J. Paul RobinsonAssociate Editors: Zbigniew Darzynkiewicz, Phillip Dean, LynnDressler, Hans Tanke and Leon Wheeless Here is the ideal laboratorymanual for investigators involved in research and diagnosticapplications of flow cytometry. Handbook of flow cytometry methodsoffers a collection of proven, basic procedures designed for use inthe flow cytometry laboratory. Methods implemented at PurdueUniversity Cytometry Laboratories and developed in laboratories ofcontributors worldwide provide practical, step-by-step proceduresfor:* the preparation and handling of standards,reagents andspecimens* laboratory analyses for immunophenotyping* measurement of DNA content and cell cycle analyses* determination of cell physiology and function* set up and operation of flow cytometers* computational and data analysis techniques* safety precautionsDrawing on their collective experience at leading research centers,the editors have fashioned a unique "working manual" intended toserve as the starting point for the evaluation and establishment oflaboratory benchmark standards in flow and image cytometry. Eachmethod is described in detail to be used and modified depending onthe user's needs. Special features of Handbook of Flow CytometryMethods include:* ample space throughout for personal annotations* suggestions of alternative procedures; trouble-shootingtips* extensive list of reagents and suppliers* comb-bound format for ease of use at the lab benchHandbook of Flow Cytometry Methods is an essential laboratoryresource for students, faculty, and researchers in cell andmolecular biology, immunology, cancer biology, hematology/oncology,and laboratory medicine.

This book highlights the current state of the art in single cell analysis, an area that involves many fields of science – from clinical hematology, functional analysis and drug screening, to platelet and microparticle analysis, marine biology and fundamental cancer research.