Jan-thorsten Schantz - Böcker

As part of the boom in biotechnology, particularly in tissue engineering, primary human cell culture has become a major pillar in academic research and the biopharmaceutical industry. Obtaining a viable culture from a tissue sample and maintaining it for experimental, diagnostic or therapeutic purposes can be quite a challenge. Based on laboratory protocols and practical experience from many years of primary cell culture, this manual presents the basic steps necessary for culturing primary human cells.Written by students for students, the manual serves well as a practical guide to primary human cell culture. The authors have left much space for notes and the design of the manual is such that it can be continuously upgraded and extended. The content of this manual is by no means exhaustive. Protocols for specific cell types, out of over 200 different cell types in the human body, were selected from major tissue groupings in the body. They should serve as a foundation for individual researchers to experiment, explore, and establish niche protocols for their specific needs. Inspired by the practical clinical checklists available to residents and trainees in medicine, the authors have chosen a compact physical format that can fit into the pocket of a lab coat.

The second volume of the series Manuals in Biomedical Research, this book is aimed to be both a concise introduction to the diverse field of microscopy and a practical guide those who require the use of microscopic for methods in their research. It provides young as well as experienced scientists a state-of-the-art multidisciplinary overview of microscopic techniques, covering all the major microscopy fields in biomedical sciences and showing their application in evaluating samples ranging from molecules to cells and tissues.Microscopy has revolutionized our understanding of biological events. Within the last two decades, microscopic techniques have provided insights into the dynamics of biological processes that regulate such events. Biological discovery, to a large extent, depends on advances in imaging techniques and various microscopic techniques have emerged as central and indispensable tools in the biomedical sciences.The four authors bring with them extensive experiences spanning across disciplines such as Microbiology, Molecular and Cell Biology, Tissue Engineering, Biomedical and Regenerative Medicine and so forth, reinforcing the fact that microscopy has proven useful in countless investigations into the mysteries of life.

This manual is designed to serve as a practical guide to primary human cell culture, which is integral in both academic and industrial biotechnology research. As in the first edition, the content of the manual is not exhaustive, but rather contains selected protocols for specific cell types from major tissue groupings in the body. This improved second edition also includes a new section on stem cells and additional material on transfection. It should serve as a foundation for individual researchers to experiment, explore, and establish niche protocols for their specific needs. With its compact physical format that makes it portable and flexible for usage in a laboratory setting, the manual will be a useful guide for all beginners in primary human cell culture work.

Regenerative medicine is a fast developing field which has led to a paradigm shift in treatment of various diseases. Clinician-scientists worldwide constantly develop novel approaches in various medical specialties (surgery, internal medicine, oncology, neurology, gynecology, pediatrics, etc.) using gene therapy approaches, innovative biomaterials or stem cell based therapies.The book provides a comprehensive overview about current therapies in regenerative medicine. Like the previous volumes within the series it will cover regenerative therapies in various medical fields. A special chapter will focus on ways to commercialize regenerative therapies. The authors are again leaders in this respective fields and are experienced clinicians.

The emerging field of regenerative medicine has led to a paradigm shift in therapeutic procedures. Scientific discovery in stem cell biology and material sciences, as well as in genetics have resulted in clinical concepts that focus on regeneration rather than repair. Also, translational research provided mankind with therapeutic tools to grow complex tissues and organs for transplantation into patients. These new technologies not only benefited patients but they also have significant socioeconomic potential.This manual aims to provide an overview on a variety of clinically applied strategies in the current field of regenerative medicine, and it also contains concise key data for a rapidly growing industry. As such, both patients and doctors will find the information contained within this manual to be useful and relevant. The editors are both international leaders in the field of regenerative medicine, and both possess a broad spectrum of experience from basic research to clinical application and commercialization.