Jeremy Sanderson – författare

This handbook introduces the new microscope user to their instrument. The light microscope is described, with practical advice given on how to set up, adjust and operate the instrument. Microscopes used in both the life sciences and materials sciences are described, along with the various techniques that microscopists routinely use. Sufficient theory is included to enable the reader to understand how a high-quality image is formed and acquired to support experimental hypotheses. This book is also intended for anyone who needs to work with core imaging facility staff to easily acquire scientifically rigorous images. The book is written by an imaging facility manager with many years of experience teaching students how to set up and operate light microscopes. Advice is also given on maintaining the light microscope and cleaning objectives. Chapter 1 introduces the microscope; Chapter 2 explains how to set up the light microscope; Chapters 3-7 describe contrast enhancement methods, including using reflected-light, polarised light, and fluorescence. Chapter 8 explains how to record images, and Chapter 9 describes the optical principles underlying image formation. This book is a modern update of the first of the Royal Microscopical Society's Handbook series. Based upon the teaching and practical experience of the author, it is a complete volume of everything a student or scientist new to microscopy needs to know.

This handbook introduces the new microscope user to their instrument. The light microscope is described, with practical advice given on how to set up, adjust and operate the instrument. Microscopes used in both the life sciences and materials sciences are described, along with the various techniques that microscopists routinely use. Sufficient theory is included to enable the reader to understand how a high-quality image is formed and acquired to support experimental hypotheses. This book is also intended for anyone who needs to work with core imaging facility staff to easily acquire scientifically rigorous images. The book is written by an imaging facility manager with many years of experience teaching students how to set up and operate light microscopes. Advice is also given on maintaining the light microscope and cleaning objectives. Chapter 1 introduces the microscope; Chapter 2 explains how to set up the light microscope; Chapters 3-7 describe contrast enhancement methods, including using reflected-light, polarised light, and fluorescence. Chapter 8 explains how to record images, and Chapter 9 describes the optical principles underlying image formation. This book is a modern update of the first of the Royal Microscopical Society's Handbook series. Based upon the teaching and practical experience of the author, it is a complete volume of everything a student or scientist new to microscopy needs to know.

Introduces readers to the enlightening world of the modern light microscopeThere have been rapid advances in science and technology over the last decade, and the light microscope, together with the information that it gives about the image, has changed too. Yet the fundamental principles of setting up and using a microscope rests upon unchanging physical principles that have been understood for years. This informative, practical, full-colour guide fills the gap between specialised edited texts on detailed research topics, and introductory books, which concentrate on an optical approach to the light microscope. It also provides comprehensive coverage of confocal microscopy, which has revolutionised light microscopy over the last few decades. Written to help the reader understand, set up, and use the often very expensive and complex modern research light microscope properly, Understanding Light Microscopy keeps mathematical formulae to a minimum—containing and explaining them within boxes in the text. Chapters provide in-depth coverage of basic microscope optics and design; ergonomics; illumination; diffraction and image formation; reflected-light, polarised-light, and fluorescence microscopy; deconvolution; TIRF microscopy; FRAP & FRET; super-resolution techniques; biological and materials specimen preparation; and more. Gives a didactic introduction to the light microscopeEncourages readers to use advanced fluorescence and confocal microscopes within a research institute or core microscopy facilityFeatures full-colour illustrations and workable practical protocolsUnderstanding Light Microscopy is intended for any scientist who wishes to understand and use a modern light microscope. It is also ideal as supporting material for a formal taught course, or for individual students to learn the key aspects of light microscopy through their own study.