Jesús Jiménez-Barbero – författare

This volume explores the latest advancements in the field of Nuclear Magnetic Resonance (NMR) and discusses how new studies provide insight into the structure, conformation, dynamics, and interactions of glycoproteins. The chapters in this book covers topics such as the preparation of isotope-labeled eukaryotic glycoproteins; applications of paramagnetic NMR to dissect glycosaminoglycan-protein interactions; interactions of mucin glycoproteins; applications of NMR to decipher the interactions between viral proteins and the glycans on host cells; NMR methods to identify biomarkers based on the glycoprotein signals directly acquired from intact biofluids; and the applications of MD simulations and other computational methods to characterize the conformation and dynamics of glycoproteins. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and thorough, NMR of Glycoproteins: Methods and Protocols is a valuable resource for both new and experienced researchers who want to learn more about this important and developing field.

This volume explores the latest advancements in the field of Nuclear Magnetic Resonance (NMR) and discusses how new studies provide insight into the structure, conformation, dynamics, and interactions of glycoproteins. The chapters in this book covers topics such as the preparation of isotope-labeled eukaryotic glycoproteins; applications of paramagnetic NMR to dissect glycosaminoglycan-protein interactions; interactions of mucin glycoproteins; applications of NMR to decipher the interactions between viral proteins and the glycans on host cells; NMR methods to identify biomarkers based on the glycoprotein signals directly acquired from intact biofluids; and the applications of MD simulations and other computational methods to characterize the conformation and dynamics of glycoproteins. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and thorough, NMR of Glycoproteins: Methods and Protocols is a valuable resource for both new and experienced researchers who want to learn more about this important and developing field.

In recent years there has been increasing evidence of the importance of carbohydrates and glycoconjugates in biomedical applications, and the use of synthetic ligands based on carbohydrates as drugs has received much attention. Focussing on drug discovery from key targets and placing an emphasis on the multi-disciplinary approaches necessary to challenge these issues, this book comprehensively covers the new and recent discoveries in the area of carbohydrate drug discovery.Carbohydrates in Drug Design and Discovery is split into five sections, beginning with a introduction and perspective on the current market. The book then goes on to discuss new synthetic methods in glycobiology, the use of glycobiology in chemical biology and glycobiology in drug discovery.Providing a worldwide perspective on this broad area, and providing examples of therapeutics already developed using these methods, this book provides a comprehensive introduction, discussion and update on this fast developing field for medicinal chemists and biochemists working in industry and academia.