Joel F. Habener – författare

The purpose of this book is to bring together the many different aspects of and recent discoveries in the understanding of pancreatic endocrine development and its close links to endocrine cell physiology and dysfunction in diabetes. The intention is to combine the knowledge from clinical observations in certain diabetic syndromes with the rapidly growing insights derived from basic molecular biological investigation. At the end of the book, future impacts of these studies are outlined in which the knowledge that is gained from basic studies will be translated into new forms of treatment for diabetes. It is hoped that this book will serve established investigators, newcomers to the diabetes research community, and clinicians interested in the molecular basis of the syndromes that they encounter.

The peptide hormones are small proteins that regulate cellular metabolism through their specific interactions with tissues of the endocrine, nervous, and immune systems, as well as in embry­ onic development. During the past ten years, refinements in the techniques of recombinant DNA technology have resulted in the cloning of genes encoding approximately 50 different hormonal and regulatory peptides, including those in which the peptides themselves and the mRNAs encoding the peptides are present in only trace amounts in the tissues of origin. In addition to provid­ ing the coding sequences of recognized hormonal and regulatory peptides, gene sequencing has uncovered new bioactive peptides encoded in the precursor pro hormones that are then liberated along with the hormonal peptides during cellular cleavages of the precursors. The encoding of multiple peptides in a single mono­ cistronic mRNA appears to be a genetic mechanism for the gener­ ation of biologic diversification without requiring amplification of gene sequences. Two of the objectives in the assembly of this book are to pre­ sent, in one volume, the known primary structures of the genes encoding several of the polypeptide hormones and related regulatory peptides, and to provide an account of the various ap­ proaches that have been used to identify and select the cloned genes encoding these polypeptides. The contents of the two in­ troductory chapters are intended to provide the reader with a brief background of the approaches to gene cloning and the struc­ ture and expression of hormone-encoding genes.

The peptide hormones are small proteins that regulate cellular metabolism through their specific interactions with tissues of the endocrine, nervous, and immune systems, as well as in embry­ onic development. During the past ten years, refinements in the techniques of recombinant DNA technology have resulted in the cloning of genes encoding approximately 50 different hormonal and regulatory peptides, including those in which the peptides themselves and the mRNAs encoding the peptides are present in only trace amounts in the tissues of origin. In addition to provid­ ing the coding sequences of recognized hormonal and regulatory peptides, gene sequencing has uncovered new bioactive peptides encoded in the precursor pro hormones that are then liberated along with the hormonal peptides during cellular cleavages of the precursors. The encoding of multiple peptides in a single mono­ cistronic mRNA appears to be a genetic mechanism for the gener­ ation of biologic diversification without requiring amplification of gene sequences. Two of the objectives in the assembly of this book are to pre­ sent, in one volume, the known primary structures of the genes encoding several of the polypeptide hormones and related regulatory peptides, and to provide an account of the various ap­ proaches that have been used to identify and select the cloned genes encoding these polypeptides. The contents of the two in­ troductory chapters are intended to provide the reader with a brief background of the approaches to gene cloning and the struc­ ture and expression of hormone-encoding genes.