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Molecular toxicology is an emerging discipline that utilizes molecular and cell biology to understand how drugs and chemicals result in their unwanted effects. PCR Protocols in Molecular Toxicology is a practical guide to the use of polymerase chain reaction (PCR) to help examine, on a molecular and cellular level, how toxic responses are manifested. It offers a basic understanding of PCR and its optimization, as well as describing specific, high-impact areas of molecular toxicology and recent advances. The following techniques are described in detail:Quantitative reverse transcriptase PCR and methods to examine gene expression Differential display cloning Cloning and library screening by PCR Genotype and polymorphism analysis of drug and toxicant metabolizing enzymes Basic, non-PCR based molecular biology methodsPCR Protocols in Molecular Toxicology will aid both novices and experienced PCR practitioners in using PCR to its fullest potential.
2 568 kr
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Conducting studies of natural and man-made air contaminants is a specialized research activity that draws upon skills from a variety of disciplines including toxicology, pharmacology, industrial hygiene, and environmental health. Methods in Inhalation Toxicology describes how knowledge from these disciplines is integrated into the design and conduct of inhalation studies. Each of the vital aspects of conducting these studies is discussed in detail, including the use of animal subjects and the related quality control and ethical considerations, air purification methodology, exposure atmosphere generation and characterization, inhalation exposure systems, and real-time and post-exposure biological assessments. These methods will enable you to conduct inhalation studies, easily adding any specific measures of particular interest to your research. The book covers techniques needed to:provide suitable clean air for experiments correctly generate the particles and gases being studied select the proper exposure system monitor the exposure prepare tissues for study ensure that animals do not have confounding lung diseaseThe helpful descriptions and detailed lists of suppliers of preferred equipment and materials will help you to perform valid, reproducible inhalation toxicology studies. Methods in Inhalation Toxicology is a perfect textbook for students in toxicology programs, as well as for scientists who conduct inhalation studies in both academic and industrial environments.