John W. Dolan – författare

The latest edition of the authoritative reference to HPLC High-performance liquid chromatography (HPLC) is today the leading technique for chemical analysis and related applications, with an ability to separate, analyze, and/or purify virtually any sample. Snyder and Kirkland's Introduction to Modern Liquid Chromatography has long represented the premier reference to HPLC. This Third Edition, with John Dolan as added coauthor, addresses important improvements in columns and equipment, as well as major advances in our understanding of HPLC separation, our ability to solve problems that were troublesome in the past, and the application of HPLC for new kinds of samples.This carefully considered Third Edition maintains the strengths of the previous edition while significantly modifying its organization in light of recent research and experience. The text begins by introducing the reader to HPLC, its use in relation to other modern separation techniques, and its history, then leads into such specific topics as: The basis of HPLC separation and the general effects of different experimental conditionsEquipment and detectionThe column—the "heart" of the HPLC systemReversed-phase separation, normal-phase chromatography, gradient elution, two-dimensional separation, and other techniquesComputer simulation, qualitative and quantitative analysis, and method validation and quality controlThe separation of large molecules, including both biological and synthetic polymersChiral separations, preparative separations, and sample preparationSystematic development of HPLC separations—new to this editionTroubleshooting tricks, techniques, and case studies for both equipment and chromatogramsDesigned to fulfill the needs of the full range of HPLC users, from novices to experts, Introduction to Modern Liquid Chromatography, Third Edition offers the most up-to-date, comprehensive, and accessible survey of HPLC methods and applications available.

Gradient elution demystified Of the various ways in which chromatography is applied today, few have been as misunderstood as the technique of gradient elution, which presents many challenges compared to isocratic separation. When properly explained, however, gradient elution can be less difficult to understand and much easier to use than often assumed. Written by two well-known authorities in liquid chromatography, High-Performance Gradient Elution: The Practical Application of the Linear-Solvent-Strength Model takes the mystery out of the practice of gradient elution and helps remove barriers to the practical application of this important separation technique. The book presents a systematic approach to the current understanding of gradient elution, describing theory, methodology, and applications across many of the fields that use liquid chromatography as a primary analytical tool. This up-to-date, practical, and comprehensive treatment of gradient elution:* Provides specific, step-by-step recommendations for developing a gradient separation for any sample* Describes the best approach for troubleshooting problems with gradient methods* Guides the reader on the equipment used for gradient elution* Lists which conditions should be varied first during method development, and explains how to interpret scouting gradients* Explains how to avoid problems in transferring gradient methods With a focus on the use of linear solvent strength (LSS) theory for predicting gradient LC behavior and separations by reversed-phase HPLC, High-Performance Gradient Elution gives every chromatographer access to this useful tool.

Major advances in instrumentation and technology have made high-performance liquid chromatography (HPLC) a pervasive tool in virtually all areas of chemical and biomedical research today. Despite HPLC's enormous growth, technical problems still bedevil most users at least occasionally. Based on their immensely successful short courses, Dolan and Snyder's landmark new book -Troubleshooting LC Systems-now makes available to every practicing chromatographer the authors' wealth of expertise from years of hands-on troubleshooting of real-world LC problems-problems that routinely cause crises and unproductive downtime in nearly every lab. Believing that successful troubleshooting requires an understanding of the basics, Dolan and Snyder open by setting forth all the fundamental principles of LC operation, then move on to a microscopic description of all the elements of LC devices, to lay out their optimal preventive maintenance routines, and ultimately to offer an unprecedentedly thorough treatment of each and every facet of troubleshooting.Displaying a special tutorial mastery-honed to a fineness during their ongoing teaching and consulting activities, and a well-received feature column in LC Magazine-the book's chapters cover: automation and robotics approaches to troubleshooting reactions of samples basic separation techniques valves, tubing, syringes band broadening and tailing preventive maintenance column packing and flushing individual LC modules troubleshooting principles reagents and buffering specific problems in separation ghost and vacancy peaks quantitation and calibration pumps, seals, and pistons gradient elution problems column composition concentration and blockages sample cleanup temperature and mixing effects detectors and recorders column packing and flushing. In short, Dolan and Snyder's eminently practical new guide to Troubleshooting LC Systems is an essential laboratory handbook-one that empowers expert and novice chromatographer alike to solve every sort of practical laboratory problem on a day-to-day basis. No one using an LC system should be without it!