Jonathan V. Sweedler – författare

This book describes the benefits of using a new type of solid-state multichannel detector, i.e., the charge-transfer device, as it is used for chemical imaging and analysis.About the authors: M. Bonner Denton, co-editor, is the czar of charge- transfer devices. He is currently President of the Society for Applied Spectroscopy. Jonathan Sweedler, co-editor, is the recipient of many awards recognizing his excellent contributions (publications and patents) in the field of charge-transfer device detectors The publishing of this book coincides with the introduction by numerous commercial vendors of complete spectroscopy systems which include charge-transfer devices (CTD).This book points to the potential uses of CTD. Essentially CTDs allow chemists to get a great deal more out of their instruments; they allow chemists to avoid false positive (and false negative) results.CTDs are the future way in all kinds of spectroscopic instrumentation, including Raman, atomic emission and luminescence spectroscopy, atomic source diagnostics, and planar chromatography.

Metabolomics, the global characterisation of the small molecule complement involved in metabolism, has evolved into a powerful suite of approaches for understanding the global physiological and pathological processes occurring in biological organisms. The diversity of metabolites, the wide range of metabolic pathways and their divergent biological contexts require a range of methodological strategies and techniques. Methodologies for Metabolomics provides a comprehensive description of the newest methodological approaches in metabolomic research. The most important technologies used to identify and quantify metabolites, including nuclear magnetic resonance and mass spectrometry, are highlighted. The integration of these techniques with classical biological methods is also addressed. Furthermore, the book presents statistical and chemometric methods for evaluation of the resultant data. The broad spectrum of topics includes a vast variety of organisms, samples and diseases, ranging from in vivo metabolomics in humans and animals to in vitro analysis of tissue samples, cultured cells and biofluids.

This volume discusses the latest techniques used in the diverse fields of single cell ‘omics and covers topics such as quantifying the single cell transcriptome; isolation of cells in nanoliter volumes for single cell proteomics measurements by nano-LC-MS/MS; and single cell protein characterization by immunoblotting. A wide range of methodologies are highlighted, ranging from high-yield chemical amplification to mass spectrometry and nanotechnology for the analysis of the chemical constituents of cells. In the Neuromethods series style, chapters include both in-depth overviews, as well as detailed protocols that provide the key advice from specialists you need to get successful results in your laboratory. Cutting-edge and comprehensive, Single Cell ‘Omics of Neuronal Cells is a valuable resource for experienced and novice researchers interested in learning more about this field and its future developments.

This volume discusses the latest techniques used in the diverse fields of single cell ‘omics and covers topics such as quantifying the single cell transcriptome; isolation of cells in nanoliter volumes for single cell proteomics measurements by nano-LC-MS/MS; and single cell protein characterization by immunoblotting. A wide range of methodologies are highlighted, ranging from high-yield chemical amplification to mass spectrometry and nanotechnology for the analysis of the chemical constituents of cells. In the Neuromethods series style, chapters include both in-depth overviews, as well as detailed protocols that provide the key advice from specialists you need to get successful results in your laboratory. Cutting-edge and comprehensive, Single Cell ‘Omics of Neuronal Cells is a valuable resource for experienced and novice researchers interested in learning more about this field and its future developments.

Mass spectrometry (MS) offers unmatched capabilities for the detection, characterization, and identification of a broad range of analytes. Mass spectrometry imaging (MSI) integrates MS data with information on the spatial distributions of the analytes, further enhancing the applicability of MS. In Mass Spectrometry Imaging: Principles and Protocols, expert practitioners from academia, industry, and the clinic contribute cutting-edge protocols describing the application of MSI to investigations of analyte localization in a variety of specimens, from microorganisms to plant and animal tissues. Divided into three sections, this volume presents the principles of MS, current and future trends of MSI, and qualitative and quantitative protocols to measure and identify endogenous metabolites and xenobiotics. An array of MSI approaches and technologies for characterizing peptide and protein distributions are described in detail. Written in the highly successful Methods in Molecular Biology™ series format, protocol chapters include introductions to their respective topics, lists of the necessary materials and reagents, and step-by-step, readily reproducible laboratory procedures. Also included are notes providing tips to avoid experimental pitfalls and helpful suggestions for method troubleshooting.Comprehensive and up-to-date, Mass Spectrometry Imaging: Principles and Protocols is written for scientists, biological and chemical engineers, and clinicians who are interested in applying MSI in their work and those who would benefit from having detailed experimental guidelines available in a single, convenient source.

Mass spectrometry (MS) offers unmatched capabilities for the detection, characterization, and identification of a broad range of analytes. Mass spectrometry imaging (MSI) integrates MS data with information on the spatial distributions of the analytes, further enhancing the applicability of MS. In Mass Spectrometry Imaging: Principles and Protocols, expert practitioners from academia, industry, and the clinic contribute cutting-edge protocols describing the application of MSI to investigations of analyte localization in a variety of specimens, from microorganisms to plant and animal tissues. Divided into three sections, this volume presents the principles of MS, current and future trends of MSI, and qualitative and quantitative protocols to measure and identify endogenous metabolites and xenobiotics. An array of MSI approaches and technologies for characterizing peptide and protein distributions are described in detail. Written in the highly successful Methods in Molecular Biology™ series format, protocol chapters include introductions to their respective topics, lists of the necessary materials and reagents, and step-by-step, readily reproducible laboratory procedures. Also included are notes providing tips to avoid experimental pitfalls and helpful suggestions for method troubleshooting.Comprehensive and up-to-date, Mass Spectrometry Imaging: Principles and Protocols is written for scientists, biological and chemical engineers, and clinicians who are interested in applying MSI in their work and those who would benefit from having detailed experimental guidelines available in a single, convenient source.