K.J. Towner - Böcker

The 1st International Workshop on Acinetobacter was held on 6th September, 1986, in Manchester, England, in association with the 14th International Congress of Microbiology. That occasion was so well attended and productive that there were soon discussions about how, when and where the next meeting should be held. This time, however, there was sufficient confidence to think of a more substantial meeting and to plan for the proceedings to be published. It emerged that there was wide agreement that the time was ripe to take stock of the entire biology of Acinetobacter: its occurrence and taxonomy; its molecular biology, biochemistry and physiology; its clinical importance and its industrial and commercial applications. The 2nd International Workshop on Acinetobacter took place from 6th to 7th September, 1990, at the Institut Pasteur, Paris, and was sponsored by the Federation of European Microbiological Societies. There were about 100 participants from 19 countries. The backbone of the meeting consisted of 23 plenary lectures. There were 28 posters and the meeting closed with a general discussion which went on long after the official finishing time despite all the counter-attractions of a sunny Parisian Friday afternoon. Indeed discussions continued while cruising along the Seine and while dining at the top of the Tour Montparnasse. However, the vitality and usefulness of even the most successful meeting is difficult to transmit by the printed word.

The accurate identification and typing of microbes is essential for workers active in all fields of microbiology. Many examples of modern molecular methods have up to now been concealed in the scientific and medical literature but this introductory text considers the possible applications of such methods and compares their advantages and disadvantages. This is not a "recipe" book but is intended as a first point of reference for workers seeking an introduction to these techniques. Following the general introductory chapter, each section of the book comprises an introduction to the principles of a particular method, an overview of the methodology involved, examples of applications involving each particular method, and full references to detailed methodology and other possible applications. Methods dealt with include plasmid and chromosomal fingerprinting techniques, RFLP analysis, ribotyping, polypeptide and LPS analysis, immunoblotting, multilocus enzyme analysis and the new technique of PCR typing. This book should be of interest to clinicians, research workers and postgraduates in the field of medical microbiology.

Rapid molecular identification and typing of micro-organisms is extremely important in efforts to monitor the geographical spread of virulent, epidemic or antibiotic-resistant pathogens. It has become a mainstay of integrated hospital infection control service. In addition, numerous industrial and biotechnological applications require the study of the diversity of organisms. Conventional phenotypic identification and typing methods have long been the mainstay of microbial population and epidemiological studies, but such methods often lack adequate discrimination and their use is normally confined to the group of organisms for which they were originally devised. Molecular fingerprinting methods have flourished in recent years and many of these new methods can be applied to numerous different organisms for a variety of purposes. Standardisation of these methods is vitally important. In addition, the generation of large numbers of complex fingerprint profiles requires that a computer-assisted strategy is used for the formation and analysis of databases. The purpose of this book is to describe the best fingerprinting methods that are currently available and the computer-assisted strategies that can be used for analysis and exchange of data between laboratories.

This book is dedicated to the memory of Jan Ursing (1926 - 2000), Swedish microbiologist, taxonomist and philosopher.

"...taxonomy is on the borders of philosophy because we do not know the natural continuities and discontinuities..."