Koichi Kawakami – författare

This fully updated edition introduces new tools, models, and analytic insights that position the zebrafish even more strongly as an engine of discovery for developmental and disease biology.

This fully updated edition introduces new tools, models, and analytic insights that position the zebrafish even more strongly as an engine of discovery for developmental and disease biology.

Thissecond edition details new emerging areas of zebrafish research focusing on geneticsand genomics, techniques for developing and analyzing zebrafish disease models,and methods for neuroscience. Zebrafish:Methods and Protocols, Second Edition guides readers through methods for mutagenesisand genome editing in zebrafish,  applicationsof GFP-expressing transgenic fish, techniques for cancer models, imaging of infectionand host-pathogen interactions, metabolism and transport of lipids, and  the structure and function of neural circuitsand their role in generating behavior. Written in the highlysuccessful Methods in Molecular Biology series format, chapters includeintroductions to their respective topics, lists of the necessary materials andreagents, step-by-step, readily reproducible laboratory protocols, and tips ontroubleshooting and avoiding known pitfalls.  Authoritative and practical Zebrafish: Methods and Protocols, Second Edition is a usefulcomplement to the first book for new and experienced zebrafish researcheralike.

Thissecond edition details new emerging areas of zebrafish research focusing on geneticsand genomics, techniques for developing and analyzing zebrafish disease models,and methods for neuroscience. Zebrafish:Methods and Protocols, Second Edition guides readers through methods for mutagenesisand genome editing in zebrafish,  applicationsof GFP-expressing transgenic fish, techniques for cancer models, imaging of infectionand host-pathogen interactions, metabolism and transport of lipids, and  the structure and function of neural circuitsand their role in generating behavior. Written in the highlysuccessful Methods in Molecular Biology series format, chapters includeintroductions to their respective topics, lists of the necessary materials andreagents, step-by-step, readily reproducible laboratory protocols, and tips ontroubleshooting and avoiding known pitfalls.  Authoritative and practical Zebrafish: Methods and Protocols, Second Edition is a usefulcomplement to the first book for new and experienced zebrafish researcheralike.

In the last 20 years, research activity using the zebrafish Danio rerio has increased dramatically, due in part to the ease of breeding and raising them, their genetic tractability, embryonic accessibility, and their imaging potential. In Zebrafish: Methods and Protocols, expert researchers provide a collection of experimental techniques for the genetic modification, manipulation, and in vivo live imaging of zebrafish. This volume collects sophisticated and practical techniques, including several not yet widely known, from laboratories around the world. Written in the highly successful Methods in Molecular Biology™ series format, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.Authoritative and accessible, Zebrafish: Methods and Protocols seeks to aid and inspire scientists utilizing this versatile animal model in their research.