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This third edition expands upon the previous two editions with new and updated methods presenting comprehensive, step-by-step protocols for recently developed techniques that were not included in the earlier volumes. Chapters feature contributions from the globally leading experts in the field, aiming to equip researchers with the practical tools necessary for successful CRISPR experimentation. Authoritative and cutting-edge, CRISPR-Cas Methods, Volume 3 aims to be a useful and practical guide for researches to help further their study in this field.