Louise O'Connor - Böcker

With the aim of showing how educators can use their skills to inform practical action on problematic drug issues, this text identifies the drug issues emerging in the classroom and highlights strategies for dealing with them.

Invasive fungal infections are a significant cause of morbidity and mortality. Over the past decade there has been a concerted effort to develop reliable methods for the detection of such infections. In Fungal Diagnostics: Methods and Protocols, expert researchers in the field detail the introduction of new technology into a diagnostic setting include ease of use, and rapid turnaround time without compromising sensitivity and specificity. Focusing specifically on fungal detection in clinical settings, fungal diagnostics including, environmental testing, agriculture and food production and veterinary diagnostics. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Fungal Diagnostics: Methods and Protocols seeks to aid scientists into the further study of diagnostic and detection of fungi.

The field of bacterial diagnostics has seen unprecedented advances in recent years. The increased need for accurate detection and identification of bacteria in human, animal, food, and environmental samples has fueled the development of new techniques. The field has seen extensive research aided by the information from bacterial genome sequencing projects. Although traditional methods of bacterial detection and identification remain in use in laboratories around the world, there is now a growing trend toward the use of nucleic ac- based diagnostics and alternative biochemically and immunologically based formats. The ultimate goal of all diagnostic tests is the accurate detection, identification, or typing of microorganisms in samples of interest. Although the resulting information is of obvious use in the areas of patient management, animal health, and quality control, it is also of use in monitoring routes of infection and outlining strategies for infection control. There is, therefore, a need to ensure that the information being provided is of the highest standard and that any new technique is capable of delivering this.

This fully updated edition includes the novel identification, detection, and typing technologies that are transforming the field of diagnostic testing. The authors, working at the forefront of diagnostic test development, highlight these new technologies and describe current and future molecular diagnostic tests and related nucleic acid extraction methods. The protocols range from advanced molecular detection, quantification, and typing systems, to protocols for diagnostic protein identification, serological testing, and cell culture-based assays. The emphasis is on nucleic acid-based diagnostics and alternative biochemically- and immunologically-based formats that can provide significant potential for multiparameter testing and automation.

During much of his brief and troubled life, Victor Marion Rose was a walking anomaly. The scion of a venerable Texas farming and ranching family, he was widely reported to be unable to distinguish one horse from another. He fought for the Confederacy and endured imprisonment at Ohio's notorious Camp Chase, yet he later bitterly decried the Civil War as utter folly for the South. His florid poetry often celebrated the feminine mystique and ideal as he considered it, yet he was infamously unfaithful and sometimes abusive in his relationships with women. He built a respected reputation as a journalist and historian, and at the same time, he struggled with alcoholism and bouts of deep depression.Born in 1842 as the third of thirteen children of a wealthy Victoria, Texas, planter, Victor Marion Rose served as publisher and editor of the Victoria Advocate from 1869 to 1873 before moving to Laredo—reportedly due to a scandalous love affair—where he edited the Laredo Times. He also wrote volumes of poetry and published several histories of South Texas and the biography of Gen. Ben McCulloch. Rose ultimately succumbed to pneumonia in February 1893.Louise S. O'Connor, a descendant of Victor Marion Rose, has mined family records and recorded family traditions about “Uncle Vic.” She carefully reviewed Rose's collected papers, both in her personal possession and in the archives of the Briscoe Center for American History and other repositories. Wild Rose provides an intimate portrait of a complicated individual who, despite his frequently unsuccessful struggles with his demons, nevertheless left an important mark on Texas history and letters.