Lucília Domingues – författare

This second volume focuses on PCR methods and PCR application specificities to the biotechnology and bioengineering field. New and updated chapters detail real-time PCR protocols, synthetic biology applications, pathogen detection, microfluidics, digital, multiplex detection recent advances. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.  Authoritative and cutting-edge, PCR: Methods and Protocols, Second Edition  aims to be a useful and practical guide to new researchers and experts looking to expand their knowledge.

This second volume focuses on PCR methods and PCR application specificities to the biotechnology and bioengineering field. New and updated chapters detail real-time PCR protocols, synthetic biology applications, pathogen detection, microfluidics, digital, multiplex detection recent advances. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and cutting-edge, PCR: Methods and Protocols, Second Edition  aims to be a useful and practical guide to new researchers and experts looking to expand their knowledge. 

This second volume focuses on PCR methods and PCR application specificities to the biotechnology and bioengineering field. New and updated chapters detail real-time PCR protocols, synthetic biology applications, pathogen detection, microfluidics, digital, multiplex detection recent advances. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.  Authoritative and cutting-edge, PCR: Methods and Protocols, Second Edition  aims to be a useful and practical guide to new researchers and experts looking to expand their knowledge.

This volume details PCR technique with focus on its application specificities to the biotechnology and bioengineering field.  Chapters are broken into five sections covering sgeneral PCR protocols, different applied examples to molecular and synthetic biotechnology, food science and technology, environmental microbiology and molecular ecology,  and healthcare. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge, PCR: Methods and Protocols hopes to be a valuable and useful resource for wet lab researchers, particularly within the biotechnology and bioengineering field.

This volume details PCR technique with focus on its application specificities to the biotechnology and bioengineering field.  Chapters are broken into five sections covering sgeneral PCR protocols, different applied examples to molecular and synthetic biotechnology, food science and technology, environmental microbiology and molecular ecology,  and healthcare. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge, PCR: Methods and Protocols hopes to be a valuable and useful resource for wet lab researchers, particularly within the biotechnology and bioengineering field.

This book provides a comprehensive overview of the filamentous fungus Ashbya gossypii, an emerging microbial host in industrial biotechnology. Recognized for its natural ability to overproduce riboflavin (vitamin B2), A. gossypii has evolved from a niche model organism into a promising candidate for sustainable biotechnological applications. This book synthesizes decades of foundational research and recent advances to present a complete picture of the organism’s utility in modern biosciences. The book begins with a detailed history of A. gossypii (Chapter 1), tracing its discovery, early industrial uses, and the scientific milestones that led to its current role in biotechnology. Chapter 2 covers its physiology and metabolism, highlighting the unique features of its multinucleated hyphae, aerobic metabolism, and natural biosynthetic capabilities. Chapter 3 focuses on how A. gossypii’s natural metabolism is being optimized for industrial applications. It covers cutting-edge metabolic engineering and synthetic biology strategies, showcasing how genetic tools have been used to redirect carbon flux, enhance product yields, and expand the range of biosynthesized compounds. Chapter 4 addresses bioprocessing innovations and biosustainable strategies, such as using renewable feedstocks, optimizing cultivation conditions, and improving strain robustness for industrial scalability. The final chapter (Chapter 5) offers forward-looking perspectives, identifying future opportunities and challenges in leveraging A. gossypii for green chemistry and biotechnological fields. As sustainability becomes a cornerstone of modern industry, this book positions A. gossypii as a key player in the next generation of microbial production platforms. Targeted at researchers, biotechnologists, and students in microbiology and metabolic engineering, this book is both a foundational reference and a roadmap for innovation in fungal biotechnology.