Lukas Rieder - Böcker

This volume provides readers with a useful and comprehensive resource of laboratory protocols for working with Pichia pastoris. The chapters in this book are organized into seven parts: introduction, bioinformatics, cell and systems biology, gene expression cassettes and vectors, Pichia pastoris expression strains, upstream bioprocessing, and downstream processing and product analysis. This book covers basic and newer laboratory methods, starting from the gene and genetic construct to mature (yet reasonable) amounts of a recombinant product being produced, purified, and characterized. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Cutting-edge and thorough, Pichia pastoris: Methods and Protocols is a valuable resource for both new and experienced researchers who want to learn more about this important and developing field.

Management Control is the process by which managers at all hierarchical levels ensure that their strategic intentions are realized. This requires a management control system that enables managers to map external developments to the internal planning and control processes and to improve the coordination between all actors.The book offers concrete guidance on how to build an integrated planning and control system. The requirements are derived from management models and from corporate management practice. The book presents the fundamentals and models, while also guiding readers through a comprehensive simulation model programmed in Excel. Using this model, readers can trace the dependencies, structures and calculation methods used in detail, and identify the effects on other areas. The goal is to provide a design template for the implementation of a decision-relevant management accounting system as well as for winning internal piloting indicators and early warning information that readers can use at their own organizations. Given its focus, the book will be a valuable asset for managers and specialists, service providers, project developers, producers and traders, public enterprises, NGOs, consultants and lecturers in the fields of management, controllership and information technology.