Matthew McKenzie - Böcker

This third edition is comprised of well-established protocols that are considered the gold standard in the field as well as new methodologies for mitochondrial DNA analysis. of Mitochondrial DNA: Methods and Protocols describes protocols for detecting mutations in mitochondrial DNA, techniques to assess mitochondrial DNA damage, visualization of mitochondrial DNA in situ, detection of mitochondrial DNA nucleoids within the mitochondria, methods for analyzing mitochondrial DNA replication, mitochondrial DNA-encoded protein translation and  mitochondrial DNA copy number,  the latest technologies for modifying the mitochondrial genome and methods for the purification of proteins involved in the replication and transcription of mitochondrial DNA. Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls.Authoritative and practical, Mitochondrial DNA: Methods and Protocols, Third Edition will be useful not only for mitochondrial researchers but also for scientists studying human diseases where mitochondrial DNA variation has been recognized as an important pathogenic factor, including cancer and neurodegeneration.

This third edition is comprised of well-established protocols that are considered the gold standard in the field as well as new methodologies for mitochondrial DNA analysis. of Mitochondrial DNA: Methods and Protocols describes protocols for detecting mutations in mitochondrial DNA, techniques to assess mitochondrial DNA damage, visualization of mitochondrial DNA in situ, detection of mitochondrial DNA nucleoids within the mitochondria, methods for analyzing mitochondrial DNA replication, mitochondrial DNA-encoded protein translation and  mitochondrial DNA copy number,  the latest technologies for modifying the mitochondrial genome and methods for the purification of proteins involved in the replication and transcription of mitochondrial DNA. Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls.Authoritative and practical, Mitochondrial DNA: Methods and Protocols, Third Edition will be useful not only for mitochondrial researchers but also for scientists studying human diseases where mitochondrial DNA variation has been recognized as an important pathogenic factor, including cancer and neurodegeneration.

With skillful storytelling, Matthew McKenzie weaves together the industrial, cultural, political, and ecological history of New England's fisheries through the story of how the Boston haddock fleet - one of the region's largest and most heavily industrialized - rose, flourished, and then fished itself into near oblivion before the arrival of foreign competition in 1961. This fleet also embodied the industry's change during this period, as it shucked its sail-and-oar, hook-and-line origins to embrace mechanized power and propulsion, more sophisticated business practices, and political engagement.Books, films, and the media have long portrayed the Yankee fisherman's hard-scrabble existence, as he faced brutal weather on the open seas and unnecessary governmental restrictions. As McKenzie contends, this simplistic view has long betrayed commercial fisheries' sophisticated legislative campaigns in Washington, DC, as they sought federal subsidies and relief and, eventually, fewer constricting regulations. This clash between fisheries' representation and their reality still grips fishing communities today as they struggle to navigate age-old trends of fleet consolidation, stock decline, and intense competition.