Nagwa El-Badri - Böcker

This textbook covers the basic aspects of stem cell research and applications in regenerative medicine. Each chapter includes a didactic component and a practical section. The book offers readers insights into:How to identify the basic concepts of stem cell biology and the molecular regulation of pluripotency and stem cell development.How to produce induced pluripotent stem cells (iPSCs) and the basics of transfection.The biology of adult stem cells, with particular emphasis on mesenchymal stromal cells and hematopoietic stem cells, and the basic mechanisms that regulate them. How cancer stem cells arise and metastasize, and their properties.How to develop the skills needed to isolate, differentiate and characterize adult stem The clinical significance of stem cell research and the potential problems that need to be overcome. Evaluating the use of stem cells for tissue engineering and therapies (the amniotic membrane) The applications of bio-nanotechnology in stem cell research.How epigenetic mechanisms, including various DNA modifications and histone dynamics, are involved in regulating the potentiality and differentiation of stem cells.The scientific methods, ethical considerations and implications of stem cell research.

Regenerative medicine aims to cure chronic intractable diseases by replacing malfunctioning cells and tissues with healthy ones and enhancing their repair and regeneration. Over the past decade, there has been exponential reporting of new technologies that advanced translational and clinical research in this field. Regenerative medicine applications have thus evolved to include tissue engineering and bioprinting in addition to cell therapy and gene therapy. Importantly, new technologies in regenerative medicine are increasingly moving to replace organ and tissue transplantation with cellular therapies and the use of extracellular vesicles (EVs). The latter are characterized as important vectors and therapeutic targets in many diseases such as cancer, neurodegenerative diseases, cardiovascular diseases, and inflammation. The book chapters cover new technologies and advances in regenerative medicine to treat chronic diseases, including cancer, diabetes, musculoskeletal diseases, dental diseases, and others.

The book reviews the main approaches for generation of differentiated cells from various types of stem cells, including embryonic, placental and cord blood stem cells through marrow, adipose tissue and dental pulp. The book starts with an overview of experimental protocols applied to generate insulin secreting cells, neural cells, heart cells, and other tissue specific cells ex vivo and in experimental animals. This is followed by exhaustive review of clinical trials in these pathologies. It continues with a comparison of the merits of successful transplantation in humans versus animal experimentation, and highlights the most promising clinical applications in the field. Special chapters are devoted to the topic of tissue engineering and modern synthetic and biological scaffolds. It is essential reading for scientists and researchers in tissue engineering and stem cell research as well as clinicians who are involved in developing or testing stem cell therapies.