Liposomes are cellular structures made up of lipid molecules. Important as a cellular model in the study of basic biology, liposomes are also used in clinical applications such as drug delivery and virus studies.- Methods in Liposome Preparation- Physiochemical Characterization of Liposomes

Liposomes are cellular structures made up of lipid molecules. Important as a cellular model in the study of basic biology, liposomes are also used in clinical applications such as drug delivery and virus studies.- Liposomes in Biochemistry- Liposomes in Molecular Cell Biology- Liposomes in Molecular Virology

Liposomes are cellular structures made up of lipid molecules. Important as a cellular model in the study of basic biology, liposomes are also used in clinical applications such as drug delivery and virus studies. Methods in Liposome Preparation Physiochemical Characterization of Liposomes

Liposomes are cellular structures made up of lipid molecules. Important as a cellular model in the study of basic biology, liposomes are also used in clinical applications such as drug delivery and virus studies. Liposomes in Biochemistry Liposomes in Molecular Cell Biology Liposomes in Molecular Virology

The fIrst reports on the tropism of the human immunodefIciency virus (HIV), then called human T-celilymphotrophic virus type ill (HTLY- ill) or lymphadenopathy associated virus (LA V), indicated an almost absolute specifIcity of the virus for T4 (helper/inducer) lymphocytes. It became apparent a few years later that macrophages were also infectable by HIV. The common cellular receptor for the virus on these cell types was CD4. A remarkable series of studies by fIve different groups, four of which were published in the same issue of~, showed that recombinant soluble CD4 could inhibit the infectivity of the virus. Subsequent studies began to reveal, however, that cells not expressing CD4 could also be infected, thus raising the possibility that almost any cell in the body could act as a latent reservoir for the virus. Recent observations that the infectivity of patient isolates is not inhibited at the low concentrations of rsCD4 that inhibit laboratory strains, also indicate the importance of CD4-independent mechanisms. The mechanisms of fusion of my with cellular membranes are under investigation by a large number of laboratories.These studies range from the determination of the oligomeric structure of the envelope glycoproteins, to the analysis of the function of various regions of the proteins by site-directed mutagenesis, to the fluorimetric monitoring of membrane fusion. This book originated as the proceedings of a Workshop on the Mechanisms and Specificity of HIV Entry into Host Cells, held at the University of California, San Francisco in June 1989.

This detailed volume explores the methods used for most of the recent approaches to suicide gene therapy of cancer, which exploits promoters that are specific to cancer cells, thereby ensuring (or greatly increasing the likelihood) that the therapeutic gene is expressed only in cancer cells. The book also contains chapters describing methods to improve the safety of cell therapy and techniques utilizing bone marrow mesenchymal cells. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.  Authoritative and practical, Suicide Gene Therapy: Methods and Protocols serves as an ideal guide for researchers expanding upon our knowledge and application of this vital form of cancer therapy.

This detailed volume explores the methods used for most of the recent approaches to suicide gene therapy of cancer, which exploits promoters that are specific to cancer cells, thereby ensuring (or greatly increasing the likelihood) that the therapeutic gene is expressed only in cancer cells. The book also contains chapters describing methods to improve the safety of cell therapy and techniques utilizing bone marrow mesenchymal cells. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.  Authoritative and practical, Suicide Gene Therapy: Methods and Protocols serves as an ideal guide for researchers expanding upon our knowledge and application of this vital form of cancer therapy.

This volume contains the lectures presented at the NATO Advanced Study Institute (ASI) on "Trafficking of Intracellular Membranes: From Molecular Sorting to Membrane Fusion", held in Espinho, Portugal, from June 19 to June 30,1994. The objective of this Institute was to survey recent developments and to discuss future directions in the rapidly advancing field of membrane cell biology, with particular emphasis on the dynamical properties and intracellular flow of membranes. A wide range of interrelated topics around the central theme of intracellular trafficking of membranes was covered, including lipid flow, membrane fusion, dynamics of membrane components, protein folding and assembly, vesicular transport in membrane biogenesis, exocytosis and endocytosis. A large variety of experimental techniques and systems, including the application of viruses and model systems, to study these processes was also considered. Membrane cell biology is a broad discipline which encompasses many scientific areas including cell biology, biochemistry, biophysics, virology, immunonology and genetics. Indeed, recent advances in the cell biology of membranes could not have been made without this multidisciplinary approach. Significant progress achieved during the last few years in understanding how newly synthesized lipids and proteins find their way to the cell organelles, how molecular sorting and the continuous flow of membranes allow each cellular membrane to maintain its own distinct molecular composition, and, thereby, the individuality of the various intracellular compartments, was discussed in considerable detail in this Institute.

Liposomes are cellular structures made up of lipid molecules. Important as a cellular model in the study of basic biology, liposomes are also used in clinical applications such as drug delivery and virus studies.- Methods in Liposome Preparation- Physiochemical Characterization of Liposomes

Liposomes are cellular structures made up of lipid molecules. Important as a cellular model in the study of basic biology, liposomes are also used in clinical applications such as drug delivery and virus studies.- Liposomes in Biochemistry- Liposomes in Molecular Cell Biology- Liposomes in Molecular Virology

Liposomes are cellular structures made up of lipid molecules. Important as a cellular model in the study of basic biology, liposomes are also used in clinical applications such as drug delivery and virus studies. Methods in Liposome Preparation Physiochemical Characterization of Liposomes

Liposomes are cellular structures made up of lipid molecules. Important as a cellular model in the study of basic biology, liposomes are also used in clinical applications such as drug delivery and virus studies. Liposomes in Biochemistry Liposomes in Molecular Cell Biology Liposomes in Molecular Virology

The fIrst reports on the tropism of the human immunodefIciency virus (HIV), then called human T-celilymphotrophic virus type ill (HTLY- ill) or lymphadenopathy associated virus (LA V), indicated an almost absolute specifIcity of the virus for T4 (helper/inducer) lymphocytes. It became apparent a few years later that macrophages were also infectable by HIV. The common cellular receptor for the virus on these cell types was CD4. A remarkable series of studies by fIve different groups, four of which were published in the same issue of~, showed that recombinant soluble CD4 could inhibit the infectivity of the virus. Subsequent studies began to reveal, however, that cells not expressing CD4 could also be infected, thus raising the possibility that almost any cell in the body could act as a latent reservoir for the virus. Recent observations that the infectivity of patient isolates is not inhibited at the low concentrations of rsCD4 that inhibit laboratory strains, also indicate the importance of CD4-independent mechanisms. The mechanisms of fusion of my with cellular membranes are under investigation by a large number of laboratories.These studies range from the determination of the oligomeric structure of the envelope glycoproteins, to the analysis of the function of various regions of the proteins by site-directed mutagenesis, to the fluorimetric monitoring of membrane fusion. This book originated as the proceedings of a Workshop on the Mechanisms and Specificity of HIV Entry into Host Cells, held at the University of California, San Francisco in June 1989.

This detailed volume explores the methods used for most of the recent approaches to suicide gene therapy of cancer, which exploits promoters that are specific to cancer cells, thereby ensuring (or greatly increasing the likelihood) that the therapeutic gene is expressed only in cancer cells. The book also contains chapters describing methods to improve the safety of cell therapy and techniques utilizing bone marrow mesenchymal cells. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.  Authoritative and practical, Suicide Gene Therapy: Methods and Protocols serves as an ideal guide for researchers expanding upon our knowledge and application of this vital form of cancer therapy.

This detailed volume explores the methods used for most of the recent approaches to suicide gene therapy of cancer, which exploits promoters that are specific to cancer cells, thereby ensuring (or greatly increasing the likelihood) that the therapeutic gene is expressed only in cancer cells. The book also contains chapters describing methods to improve the safety of cell therapy and techniques utilizing bone marrow mesenchymal cells. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.  Authoritative and practical, Suicide Gene Therapy: Methods and Protocols serves as an ideal guide for researchers expanding upon our knowledge and application of this vital form of cancer therapy.

This volume contains the lectures presented at the NATO Advanced Study Institute (ASI) on "Trafficking of Intracellular Membranes: From Molecular Sorting to Membrane Fusion", held in Espinho, Portugal, from June 19 to June 30,1994. The objective of this Institute was to survey recent developments and to discuss future directions in the rapidly advancing field of membrane cell biology, with particular emphasis on the dynamical properties and intracellular flow of membranes. A wide range of interrelated topics around the central theme of intracellular trafficking of membranes was covered, including lipid flow, membrane fusion, dynamics of membrane components, protein folding and assembly, vesicular transport in membrane biogenesis, exocytosis and endocytosis. A large variety of experimental techniques and systems, including the application of viruses and model systems, to study these processes was also considered. Membrane cell biology is a broad discipline which encompasses many scientific areas including cell biology, biochemistry, biophysics, virology, immunonology and genetics. Indeed, recent advances in the cell biology of membranes could not have been made without this multidisciplinary approach. Significant progress achieved during the last few years in understanding how newly synthesized lipids and proteins find their way to the cell organelles, how molecular sorting and the continuous flow of membranes allow each cellular membrane to maintain its own distinct molecular composition, and, thereby, the individuality of the various intracellular compartments, was discussed in considerable detail in this Institute.