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Traditional Chinese medicine (TCM) has been used for centuries in the Orient to relieve or treat diseases. Its efficacy is now attracting worldwide attention. Chinese medicinal materials are traditionally identified by their organoleptic characteristics, such as the texture or the odor. To ensure consistency of quality and avoid misuse, reliable programs are urgently needed for authentication. Proper authentication also assists in the detection and conservation of endangered species. DNA molecules offer a more definitive means of authentication, as genetic composition is unique for each taxon and is less affected by age, physiological conditions and environmental factors. This book is the first of its kind to introduce and evaluate the molecular techniques that have been developed to authenticate Chinese medicinal materials.The contents of the book are divided into four parts. The first part reviews the current status of molecular authentication of Chinese medicinal materials. The second part describes the equipment and materials required for the work. In the third part, researchers in the field present the principles and step-by-step protocols for a wide spectrum of techniques used in the molecular authentication of TCM. The last part provides an overview of this field.
Authentication Of Chinese Medicinal Materials By Dna Technology: Techniques And Applications
Inbunden, Engelska, 2022
2 257 kr
Skickas inom 3-6 vardagar
For centuries, Chinese medicinal materials have been used for therapeutic purposes. Chinese medicinal materials are traditionally identified by their organoleptic characteristics, such as the texture or the odor. With the advancement of DNA technology, a molecular approach has become an important tool to complement organoleptic, morphological, anatomical and chemical techniques for the authentication and quality assurance of Chinese medicinal materials.In 2002, the authors published Authentication of Chinese Medicinal Materials by DNA Technology which is the first international reference in this field. After 20 years, this new edition updates the various useful techniques and describes the new techniques developed for molecular authentication. The procedures of each DNA technique are provided in detail for 'step-by-step' experiments.This book is divided into 5 parts with 18 chapters. Part I reviews the current status of molecular authentication and introduces a wide range of DNA techniques. Part II lists the experimental procedures for molecular authentication. Part III describes the DNA fingerprinting-based techniques. Part IV describes the DNA sequencing-based techniques. Finally, Part V provides an account on the recent advancement in molecular authentication, including guidelines for setting up a proper DNA laboratory and concluding remarks.