Pauline M. Doran – författare

The emergence and refinement of techniques in molecular biology has changed our perceptions of medicine, agriculture and environmental management. Scientific breakthroughs in gene expression, protein engineering and cell fusion are being translated by a strengthening biotechnology industry into revolutionary new products and services. Many a student has been enticed by the promise of biotechnology and the excitement of being near the cutting edge of scientific advancement. However, graduates trained in molecular biology and cell manipulation soon realise that these techniques are only part of the picture. Reaping the full benefits of biotechnology requires manufacturing capability involving the large-scale processing of biological material. Increasingly, biotechnologists are being employed by companies to work in co-operation with chemical engineers to achieve pragmatic commercial goals. For many years aspects of biochemistry and molecular genetics have been included in chemical engineering curricula, yet there has been little attempt until recently to teach aspects of engineering applicable to process design to biotechnologists.This textbook is the first to present the principles of bioprocess engineering in a way that is accessible to biological scientists. Other texts on bioprocess engineering currently available assume that the reader already has engineering training. On the other hand, chemical engineering textbooks do not consider examples from bioprocessing, and are written almost exclusively with the petroleum and chemical industries in mind. This publication explains process analysis from an engineering point of view, but refers exclusively to the treatment of biological systems. Over 170 problems and worked examples encompass a wide range of applications, including recombinant cells, plant and animal cell cultures, immobilised catalysts as well as traditional fermentation systems.* * First book to present the principles of bioprocess engineering in a way that is accessible to biological scientists* Explains process analysis from an engineering point of view, but uses worked examples relating to biological systems* Comprehensive, single-authored* 170 problems and worked examples encompass a wide range of applications, involving recombinant plant and animal cell cultures, immobilized catalysts, and traditional fermentation systems* 13 chapters, organized according to engineering sub-disciplines, are groupled in four sections - Introduction, Material and Energy Balances, Physical Processes, and Reactions and Reactors* Each chapter includes a set of problems and exercises for the student, key references, and a list of suggestions for further reading* Includes useful appendices, detailing conversion factors, physical and chemical property data, steam tables, mathematical rules, and a list of symbols used* Suitable for course adoption - follows closely curricula used on most bioprocessing and process biotechnology courses at senior undergraduate and graduate levels.

This welcome new edition discusses bioprocess engineering from the perspective of biology students. It includes a great deal of new material and has been extensively revised and expanded. These updates strengthen the book and maintain its position as the book of choice for senior undergraduates and graduates seeking to move from biochemistry/microbiology/molecular biology to bioprocess engineering.- All chapters thoroughly revised for current developments, with over 200 pgs of new material, including significant new content in: Metabolic Engineering, Sustainable Bioprocessing, Membrane Filtration, Turbulence and Impeller Design, Downstream Processing, Oxygen Transfer Systems- Over 150 new problems and worked examples- More than 100 new illustrations

This welcome new edition discusses bioprocess engineering from the perspective of biology students. It includes a great deal of new material and has been extensively revised and expanded. These updates strengthen the book and maintain its position as the book of choice for senior undergraduates and graduates seeking to move from biochemistry/microbiology/molecular biology to bioprocess engineering. All chapters thoroughly revised for current developments, with over 200 pgs of new material, including significant new content in: Metabolic Engineering, Sustainable Bioprocessing, Membrane Filtration, Turbulence and Impeller Design, Downstream Processing, Oxygen Transfer Systems Over 150 new problems and worked examples More than 100 new illustrations

Bioprocess Engineering Principles, Third Edition provides a solid introduction to bioprocess engineering for students with a limited engineering background. The book explains process analysis from an engineering perspective using worked examples and problems that relate to biological systems. Application of engineering concepts is illustrated in areas of modern biotechnology, such as recombinant protein production, bioremediation, biofuels, drug development, and tissue engineering, as well as microbial fermentation. With new and expanded material, this remains the book of choice for students seeking to move into bioprocess engineering Includes more than 350 problems that demonstrate how fundamental principles are applied in areas such as biofuels, bioplastics, bioremediation, tissue engineering, site-directed mutagenesis, recombinant protein production, and drug development, as well as for traditional microbial fermentation Provides in-depth treatment of fluid flow, turbulence, mixing, and impeller design, reflecting recent advances in our understanding of mixing processes and their importance in determining the performance of cell cultures Focuses on underlying scientific and engineering principles rather than on specific biotechnology applications, providing a sound basis for teaching bioprocess engineering Presents new or expanded coverage of such topics as enzyme kinetics, downstream processing, disposable reactors, genetic engineering, and the technology of fermentation

Bioprocess Engineering Principles, Third Edition provides a solid introduction to bioprocess engineering for students with a limited engineering background. The book explains process analysis from an engineering perspective using worked examples and problems that relate to biological systems. Application of engineering concepts is illustrated in areas of modern biotechnology, such as recombinant protein production, bioremediation, biofuels, drug development, and tissue engineering, as well as microbial fermentation. With new and expanded material, this remains the book of choice for students seeking to move into bioprocess engineering- Includes more than 350 problems that demonstrate how fundamental principles are applied in areas such as biofuels, bioplastics, bioremediation, tissue engineering, site-directed mutagenesis, recombinant protein production, and drug development, as well as for traditional microbial fermentation- Provides in-depth treatment of fluid flow, turbulence, mixing, and impeller design, reflecting recent advances in our understanding of mixing processes and their importance in determining the performance of cell cultures- Focuses on underlying scientific and engineering principles rather than on specific biotechnology applications, providing a sound basis for teaching bioprocess engineering- Presents new or expanded coverage of such topics as enzyme kinetics, downstream processing, disposable reactors, genetic engineering, and the technology of fermentation

Hairy roots are plant roots that have been genetically transformed and can be cultured on a large scale. They can replace the whole plant in many research projects, and offer a range of technical advantages over plant cell cultures. Hairy roots are now used in studies of plant secondary metabolism and its genetic manipulation, as hosts for the production of foreign proteins, for plant propagation in agriculture, in environmental research, and for the development of new engineering technology for large-scale production of plant chemicals. Hairy root culture is an interdisciplinary science, with important and expansive applications. This volume is the first to be dedicated solely to the many facets of hairy root culture. The number of papers dedicated to hairy roots is rising exponentially, and with the increasing amount of research already underway this forms a timely publication. It is written and edited by acknowledged experts in the areas of hairy root culture and product synthesis, plant propagation, bio-processing and environmental aspects of hairyroots.

Hairy roots are plant roots that have been genetically transformed and can be cultured on a large scale. They can replace the whole plant in many research projects, and offer a range of technical advantages over plant cell cultures. Hairy roots are now used in studies of plant secondary metabolism and its genetic manipulation, as hosts for the production of foreign proteins, for plant propagation in agriculture, in environmental research, and for the development of new engineering technology for large-scale production of plant chemicals. Hairy root culture is an interdisciplinary science, with important and expansive applications. This volume is the first to be dedicated solely to the many facets of hairy root culture. The number of papers dedicated to hairy roots is rising exponentially, and with the increasing amount of research already underway this forms a timely publication. It is written and edited by acknowledged experts in the areas of hairy root culture and product synthesis, plant propagation, bio-processing and environmental aspects of hairyroots.

This volume aims to describe clearly and in detail the key practical skills involved in cartilage tissue engineering. Methods are outlined for isolation and expansion of chondrocytes and stem cells; differentiation; synthesis and application of three-dimensional scaffolds; design and operation of bioreactors; in vivo testing of engineered constructs; and molecular and functional analysis of cartilage cells and tissues. Frequently used technologies are covered, as well as more recent advances in inspirational areas such as ‘smart’ biomaterial development, novel bioreactor design, -omics analysis, and genetic manipulation of matrix synthesis. The key procedures discussed either underpin the progress already achieved in cartilage tissue engineering or are indicative of the direction of future research in the area. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, readily reproducible step-by-step laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge, Cartilage Tissue Engineering: Methods and Protocols is a useful resource that informs the scientific community about the experimental work covering a broad range of objectives for cartilage synthesis and regeneration.

This volume aims to describe clearly and in detail the key practical skills involved in cartilage tissue engineering. Methods are outlined for isolation and expansion of chondrocytes and stem cells; differentiation; synthesis and application of three-dimensional scaffolds; design and operation of bioreactors; in vivo testing of engineered constructs; and molecular and functional analysis of cartilage cells and tissues. Frequently used technologies are covered, as well as more recent advances in inspirational areas such as ‘smart’ biomaterial development, novel bioreactor design, -omics analysis, and genetic manipulation of matrix synthesis. The key procedures discussed either underpin the progress already achieved in cartilage tissue engineering or are indicative of the direction of future research in the area. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, readily reproducible step-by-step laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge, Cartilage Tissue Engineering: Methods and Protocols is a useful resource that informs the scientific community about the experimental work covering a broad range of objectives for cartilage synthesis and regeneration.

Victor P. Bulgakov, Yuri N. Shkryl, Galina N. Veremeichik, Tatiana Y. Gorpenchenko and Yuliya V. Vereshchagina: Recent Advances in the Understanding of Agrobacterium rhizogenes-Derived Genes and Their Effects on Stress Resistance and Plant Metabolism. Le Zhao, Guy W. Sander and Jacqueline V. Shanks: Perspectives of the Metabolic Engineering of Terpenoid Indole Alkaloids in Catharanthus roseus Hairy Roots. Jian Wen Wang and Jian Yong Wu: Effective Elicitors and Process Strategies for Enhancement of Secondary Metabolite Production in Hairy Root Cultures. Amanda R. Stiles and Chun-Zhao Liu: Hairy Root Culture: Bioreactor Design and Process Intensification. Marina Skarjinskaia, Karen Ruby, Adriana Araujo, Karina Taylor, Vengadesan Gopalasamy-Raju, Konstantin Musiychuk, Jessica A. Chichester, Gene A. Palmer, Patricia de la Rosa, Vadim Mett, Natalia Ugulava,Stephen J. Streatfield and Vidadi Yusibov: Hairy Roots as a Vaccine Productionand Delivery System. Zahwa Al-Shalabi and Pauline M. Doran: Metal Uptake and Nanoparticle Synthesis in Hairy Root Cultures.

Victor P. Bulgakov, Yuri N. Shkryl, Galina N. Veremeichik, Tatiana Y. Gorpenchenko and Yuliya V. Vereshchagina: Recent Advances in the Understanding of Agrobacterium rhizogenes-Derived Genes and Their Effects on Stress Resistance and Plant Metabolism. Le Zhao, Guy W. Sander and Jacqueline V. Shanks: Perspectives of the Metabolic Engineering of Terpenoid Indole Alkaloids in Catharanthus roseus Hairy Roots. Jian Wen Wang and Jian Yong Wu: Effective Elicitors and Process Strategies for Enhancement of Secondary Metabolite Production in Hairy Root Cultures. Amanda R. Stiles and Chun-Zhao Liu: Hairy Root Culture: Bioreactor Design and Process Intensification. Marina Skarjinskaia, Karen Ruby, Adriana Araujo, Karina Taylor, Vengadesan Gopalasamy-Raju, Konstantin Musiychuk, Jessica A. Chichester, Gene A. Palmer, Patricia de la Rosa, Vadim Mett, Natalia Ugulava,Stephen J. Streatfield and Vidadi Yusibov: Hairy Roots as a Vaccine Productionand Delivery System. Zahwa Al-Shalabi and Pauline M. Doran: Metal Uptake and Nanoparticle Synthesis in Hairy Root Cultures.