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Written for both novices and experts, this text describes modern tissue culture methods within a conceptual framework of cell biology, genetics, nutrition, endocrinology, and physiology. The book's main contribution is its rendering of technical cell culture information in a comprehensive, logical format. Troubleshooting problems are approached from a theoretical perspective, helping the reader to understand and surpass the examples given, while exercises and techniques stimulate thought about the broader aspects of cell culture. Nearly 100 illustrations, including 41 halftones, highlight the book. Appendices include references to relevant Internet sites and other useful sources of information.