This volume looks at the latest techniques used in the study of Rift Valley Fever Virus (RVFV). The chapters in this book cover topics such as molecular biology, biochemistry, and cell biology approaches that enable the mechanistic study of virus-host interactions; vector competence, detection, and diagnosis; virus inactivation for biosafety purposes; virus and protein production for structural studies; high-throughput screening based on CRISPR-Cas9 technology; single-molecule RNA imaging; and physiological infection models, animal models, and immunology. Written in the high successful Methods in Molecular Biology format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Cutting-edge and comprehensive, Rift Valley Fever Virus: Methods and Protocols is a valuable resource for both novice and experts researchers who want to learn more about the important and developing field of RVFV.

This volume looks at the latest techniques used in the study of Rift Valley Fever Virus (RVFV). The chapters in this book cover topics such as molecular biology, biochemistry, and cell biology approaches that enable the mechanistic study of virus-host interactions; vector competence, detection, and diagnosis; virus inactivation for biosafety purposes; virus and protein production for structural studies; high-throughput screening based on CRISPR-Cas9 technology; single-molecule RNA imaging; and physiological infection models, animal models, and immunology. Written in the high successful Methods in Molecular Biology format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Cutting-edge and comprehensive, Rift Valley Fever Virus: Methods and Protocols is a valuable resource for both novice and experts researchers who want to learn more about the important and developing field of RVFV.