Ramnath Elaswarapu – författare

Genomics research has made significant advances in recent years. In this book, a team of internationally-renowned researchers share the most up-to-date information in a field that has in recent years switched emphasis from gene identification to functional genomics and the characterization of genes and gene products. This volume approaches this complex subject with a broad perspective to supply the reader with a vital overview of genomics and its derivative fields, with a focus on pivotal issues such as data analysis. Expansive and current, this book is a comprehensive research guide that describes both the key new techniques and more established methods. Every chapter discusses the merits and limitations of the various approaches and then provides selected tried-and-tested protocols, as well as a plethora of good practical advice for immediate use at the bench. Key features: Provides a broad introduction to current practices and techniques for lab-based research in genomicsExplains clearly and precisely how to carry out selected techniques in addition to background information on the various approachesChapters are written by a leading international authorities in the field and cover both well-known and new, tried and tested, methods for working in genomicsIncludes troubleshooting guide and reviews of alternative techniquesAn essential laboratory manual for students and researchers at all levels

Genomics research has made significant advances in recent years. In this book, a team of internationally-renowned researchers share the most up-to-date information in a field that has in recent years switched emphasis from gene identification to functional genomics and the characterization of genes and gene products. This volume approaches this complex subject with a broad perspective to supply the reader with a vital overview of genomics and its derivative fields, with a focus on pivotal issues such as data analysis. Expansive and current, this book is a comprehensive research guide that describes both the key new techniques and more established methods. Every chapter discusses the merits and limitations of the various approaches and then provides selected tried-and-tested protocols, as well as a plethora of good practical advice for immediate use at the bench.

Key features:

Provides a broad introduction to current practices and techniques for lab-based research in genomics Explains clearly and precisely how to carry out selected techniques in addition to background information on the various approaches Chapters are written by a leading international authorities in the field and cover both well-known and new, tried and tested, methods for working in genomics Includes troubleshooting guide and reviews of alternative techniques An essential laboratory manual for students and researchers at all levels

Since the first edition, published in 2001, genomics research has taken great strides. In Genomics Protocols, 2nd Edition, a team of expert researchers share the most current information in a field that has recently switched emphasis from gene identification to functional genomics and the characterization of genes and gene products. This volume approaches its complex subject with a broad perspective to supply its reader with a vital overview of genomics and its derivative fields, with a focus on pivotal issues such as data analysis. Following the highly successful Methods in Molecular Biology™ series format, the chapters provide easy-to-follow laboratory protocols, lists of the necessary materials, and the excellent Notes section, which offers tips on troubleshooting and avoiding known pitfalls. Expansive and current, Genomics Protocols, 2nd Edition is the perfect update for researchers working in this integral, growing field.

We must unashamedly admit that a large part of the motivation for editing Genomics Protocols was selfish. The possibility of assembling in a single volume a unique and comprehensive collection of complete protocols, relevant to our work and the work of our colleagues, was too good an opportunity to miss. We are pleased to report, however, that the outcome is something of use not only to those who are experienced practitioners in the genomics field, but is also valuable to the larger community of researchers who have recognized the potential of genomics research and may themselves be beginning to explore the technologies involved. Some of the techniques described in Genomics Protocols are clearly not restricted to the genomics field; indeed, a prerequisite for many procedures in this discipline is that they require an extremely high throughput, beyond the scope of the average investigator. However, what we have endeavored here to achieve is both to compile a collection of procedures concerned with geno- scale investigations and to incorporate the key components of “bottom-up” and “top-down” approaches to gene finding. The technologies described extend from those traditionally recognized as coming under the genomics umbrella, touch on proteomics (the study of the expressed protein complement of the genome), through to early therapeutic approaches utilizing the potential of genome programs via gene therapy (Chapters 27–30).

Since the first edition, published in 2001, genomics research has taken great strides. In Genomics Protocols, 2nd Edition, a team of expert researchers share the most current information in a field that has recently switched emphasis from gene identification to functional genomics and the characterization of genes and gene products. This volume approaches its complex subject with a broad perspective to supply its reader with a vital overview of genomics and its derivative fields, with a focus on pivotal issues such as data analysis. Following the highly successful Methods in Molecular Biology™ series format, the chapters provide easy-to-follow laboratory protocols, lists of the necessary materials, and the excellent Notes section, which offers tips on troubleshooting and avoiding known pitfalls. Expansive and current, Genomics Protocols, 2nd Edition is the perfect update for researchers working in this integral, growing field.