In Vitro Culture of Higher Plants presents an up-to-date and wide- ranging account of the techniques and applications, and has primarily been written in response to practical problems. Special attention has been paid to the educational aspects. Typical methodological aspects are given in the first part: laboratory set-up, composition and preparation of media, sterilization of media and plant material, isolation and (sub)culture, mechanization, the influence of plant and environmental factors on growth and development, the transfer from test-tube to soil, aids to study. The question of why in vitro culture is practised is covered in the second part: embryo culture, germination of orchid seeds, mericloning of orchids, production of disease-free plants, vegetative propagation, somaclonal variation, test-tube fertilization, haploids, genetic manipulation, other applications in phytopathology and plant breeding, secondary metabolites.

In November 1990 Indo-American Hybrid Seeds (IAHS), one of the largest and very innovative horticultural enterprises of its kind in India, celebrated its silver jubilee year in the town of Bangalore, India. On the occasion of this silver jubilee of IAHS an International Seminar on 'New Frontiers in Horticulture' was organized from 25-28th of November 1990 at the Ashok Radisson Hotel in Bangalore. IAHS was almost fully responsible in terms of organization and financially for this International Seminar. Assisted by an International Scientific Advisory Board, the organizing committee, all members of the company IAHS, really did a great job. I would like to thank in particular Mr. Mammohan Attavar (the company's founder) and Mr. Sri N.K. Bhat (partner of the company), respectively chairman and treasurer of the organizing committee, for their organizational and financial support in organizing this conference. Very special words of thanks go to my colleague editor, Dr. Jitendra Prakash, Secretary Organizing committee and Director of Biotechnology - IAHS, who was really the spill in the whole organization of our very successful conference.

In November 1990 Indo-American Hybrid Seeds (IAHS), one of the largest and very innovative horticultural enterprises of its kind in India, celebrated its silver jubilee year in the town of Bangalore, India. On the occasion of this silver jubilee of IAHS an International Seminar on ''New Frontiers in Horticulture'' was organized from 25-28th of November 1990 at the Ashok Radisson Hotel in Bangalore. IAHS was almost fully responsible in terms of organization and financially for this International Seminar. Assisted by an International Scientific Advisory Board, the organizing committee, all members of the company IAHS, really did a great job. I would like to thank in particular Mr. Mammohan Attavar (the company''s founder) and Mr. Sri N.K. Bhat (partner of the company), respectively chairman and treasurer of the organizing committee, for their organizational and financial support in organizing this conference. Very special words of thanks go to my colleague editor, Dr. Jitendra Prakash, Secretary Organizing committee and Director of Biotechnology - IAHS, who was really the spill in the whole organization of our very successful conference.

In Vitro Culture of Higher Plants presents an up-to-date and wide- ranging account of the techniques and applications, and has primarily been written in response to practical problems. Special attention has been paid to the educational aspects. Typical methodological aspects are given in the first part: laboratory set-up, composition and preparation of media, sterilization of media and plant material, isolation and (sub)culture, mechanization, the influence of plant and environmental factors on growth and development, the transfer from test-tube to soil, aids to study. The question of why in vitro culture is practised is covered in the second part: embryo culture, germination of orchid seeds, mericloning of orchids, production of disease-free plants, vegetative propagation, somaclonal variation, test-tube fertilization, haploids, genetic manipulation, other applications in phytopathology and plant breeding, secondary metabolites.

In November 1990 Indo-American Hybrid Seeds (IAHS), one of the largest and very innovative horticultural enterprises of its kind in India, celebrated its silver jubilee year in the town of Bangalore, India. On the occasion of this silver jubilee of IAHS an International Seminar on 'New Frontiers in Horticulture' was organized from 25-28th of November 1990 at the Ashok Radisson Hotel in Bangalore. IAHS was almost fully responsible in terms of organization and financially for this International Seminar. Assisted by an International Scientific Advisory Board, the organizing committee, all members of the company IAHS, really did a great job. I would like to thank in particular Mr. Mammohan Attavar (the company's founder) and Mr. Sri N.K. Bhat (partner of the company), respectively chairman and treasurer of the organizing committee, for their organizational and financial support in organizing this conference. Very special words of thanks go to my colleague editor, Dr. Jitendra Prakash, Secretary Organizing committee and Director of Biotechnology - IAHS, who was really the spill in the whole organization of our very successful conference.

In November 1990 Indo-American Hybrid Seeds (IAHS), one of the largest and very innovative horticultural enterprises of its kind in India, celebrated its silver jubilee year in the town of Bangalore, India. On the occasion of this silver jubilee of IAHS an International Seminar on ''New Frontiers in Horticulture'' was organized from 25-28th of November 1990 at the Ashok Radisson Hotel in Bangalore. IAHS was almost fully responsible in terms of organization and financially for this International Seminar. Assisted by an International Scientific Advisory Board, the organizing committee, all members of the company IAHS, really did a great job. I would like to thank in particular Mr. Mammohan Attavar (the company''s founder) and Mr. Sri N.K. Bhat (partner of the company), respectively chairman and treasurer of the organizing committee, for their organizational and financial support in organizing this conference. Very special words of thanks go to my colleague editor, Dr. Jitendra Prakash, Secretary Organizing committee and Director of Biotechnology - IAHS, who was really the spill in the whole organization of our very successful conference.