Robert Hawley – Författare

New results and interpretations challenging the notion of a uniform, macroregional collapse throughout the Late Bronze Age Eastern MediterraneanAncient Western Asia Beyond the Paradigm of Collapse and Regeneration (1200–900 BCE) presents select essays originating in a two-year research collaboration between New York University and Paris Sciences et Lettres. The contributions here offer new results and interpretations of the processes and outcomes of the transition from the Late Bronze Age to the Iron Age in three broad regions: Anatolia, northern Mesopotamia, and the Levant. Together, these challenge the notion of a uniform, macroregional collapse throughout the Eastern Mediterranean, followed by the regeneration of political powers. Current research on newly discovered or reinterpreted textual and material evidence from Western Asia instead suggests that this transition was characterized by a diversity of local responses emerging from diverse environmental settings and culture complexes, as evident in the case studies collected here in history, archaeology, and art history. The editors avoid particularism by adopting a regional organization, with the aim of identifying and tracing similar processes and outcomes emerging locally across the three regions. Ultimately, this volume reimagines the Late Bronze–Iron Age transition as the emergence of a set of recursive processes and outcomes nested firmly in the local cultural interactions of western Asia before the beginning of the new, unifying era of Assyrian imperialism.

With rapid improvements in instrumentation, lasers, fluorophores, and data analysis software, flow cytometry is riding the crest of unprecedented, innovative advances. This thoroughly revised and up-to-date third edition of Flow Cytometry Protocols highlights the expanding contribution of flow cytometry to basic biological research and diagnostic medicine. Written by leading experts in the field, the book presents cutting-edge topics such as polychromatic, quantitative, and high throughput flow cytometry, novel multiparametric data analysis which breaks the dimensionality barrier, standard practice and safety measures for aerosol-generating cell sorting, conventional and imaging flow cytometry as well as minimalist imaging cytometry. As a volume in the highly successful Methods in Molecular Biology™ series, chapters contain brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and extensive notes on troubleshooting and avoiding known pitfalls.Authoritative and comprehensive, Flow Cytometry Protocols, Third Edition presents established as well as new flow cytometric methodologies in order to introduce beginning users to basic applications while opening new avenues of innovation for seasoned users.

This thoroughly revised and updated edition of a widely used practical guide to flow cytometry describes in step-by-step detail an array of time proven and cutting-edge techniques much needed in today's advanced laboratories. These readily reproducible methods deploy emerging flow cytometry technologies in many new applications, especially in the field of stem cells, functional genomics and proteomics, and microbiology. Here, the aspiring investigator will find methods for the characterization of stem/progenitor cells by monitoring the efflux of fluorescent dyes and the elucidation of signal transduction pathways using phospho-specific antibodies. There are also techniques for monitoring gene transfer and expression using fluorescent protein technology, high throughput screening for discovery of novel protein interactions, phenotypic and functional characterization of T cell subsets and precursors, and microbial flow cytometry, to highlight but some of the many useful procedures.