Sidney A. Katz - Böcker

Bringing together the recent and relevant contributions of over 125 scientists from industry, government, and academia in North America and Western Europe, Alternative Toxicological Methods explores the development and validation of replacement, reduction, and refinement alternatives (the 3Rs) to animal testing. Internationally recognized scientists present what has been accomplished thus far in developing acceptable alternatives to traditional animal toxicological assessment and provide potentially new initiatives.In over 40 chapters, the book addresses 7 themes, beginning with the validation and regulatory acceptance of alternatives in the US, the UK, and Western Europe. Then the book covers methods based on the mechanisms of eye irritation at the ocular surface, dermal toxicity testing, and assessing neurotoxicity. It also includes a case study in the use of alternatives to determine the mechanism of sulfur mustard action and discusses the role of transgenics and toxicokinetics in the development of alternative toxicity tests. Finally the book presents recent innovations in alternatives, including the use of archival data, and in silico techniques.Focusing on the science and not the politics, Alternative Toxicological Methods concisely discusses the application of state-of-the-art methods and explores cutting-edge research related to developing and validating alternatives to animal testing. This book will benefit toxicologists, cosmetic chemists, pharmacologists, experimental biologists, dermatologists, ophthalmologists, and regulatory officers in their quest for applying the 3Rs to the use of animals in toxicological testing of drug candidates, chemicals, and cosmetics.

Internationally known scientists review what has been accomplished thus far in developing acceptable alternatives to classical animal toxicity assessment methods. Authoritative chapters describe not only the many practical alternative approaches that have recently emerged, but also consider current trends and likely future approaches, as well as emerging automated assessment techniques. Hazard assessment applications and the validation of new alternative methods are also discussed. The methods presented here will help toxicologists, cosmetic chemists, and pharmacologists greatly reduce, refine, or even possibly replace, the use of laboratory animals in their work. Life science researchers will also find many new opportunities for detecting endocrine disruptions. Regulatory officers will gain a new perspective on alternative assessment, environmental assessment, and risk assessment. Significantly extending the efforts of the National Institutes of Health, the European Union, and the ICCVAM, Toxicity Assessment Alternatives offers all those evaluating toxicity today a cutting-edge collection of practical and highly effective alternatives.

The lungs provide a significant opportunity for the introduction of both therapeutic and toxic chemicals into the human body. In occupational and domestic environments, hazardous chemicals can enter the body through the lungs via gases, aerosols, and particulates from natural and anthropogenic sources. Fully updated with new research and discoveries since the last edition, Inhalation Toxicology, Third Edition presents contributions from internationally recognized scientists in the academic, commercial/industrial, and governmental sectors. A pragmatic resource for practicing professionals and students, the book comprehensively examines the relationship between the respiratory system and the toxicology of inhaled substances. Topics include: Regulatory aspects of exposure and testing Testing equipment and procedures Respiratory allergy and irritation of the respiratory tract Risk assessment Toxicology theory Toxicology modeling Toxic effects of some individual toxicantsNew topics in this third edition include collection and characterization of airborne particulate matter, the inhalation toxicology of asbestos fibers and nanoparticles, and the development of lung-on-a-chip technology for predicting in vivo responses. Each chapter concludes with thought-provoking questions and answers, enhancing the book’s educational utility.

Experiments in Nuclear Science is an introductory-level laboratory manual providing hands-on opportunities for developing insights into the origins and properties of nuclear radiations, their interactions with matter, their detection and measurement, and their applications in the physical and life sciences. Based on experiments successfully performed by hundreds of students at Rutgers University and the University of Wisconsin, this manual can be used as a stand-alone volume or alongside a textbook such as Introduction to Nuclear Science by Jeff C. Bryan.Relevant to a range of coursesEach of the 32 exercises includes an overview of the scientific phenomenon, instructions for conducting the experiments and recording the data, directions for analyzing the data and reporting the results, specific questions relating to the experiments, and several problems relating to the scientific phenomena being investigated. Validated for safety and pedagogy in the undergraduate instructional laboratory, the exercises can be used in an undergraduate course in nuclear science. Individual exercises can also be adopted to demonstrate fundamental principles in a general science course as well as introductory biology and chemistry courses. Making use of off-the-shelf instrumentation, these exercises can be performed in a conventional laboratory under the supervision of an experienced instructor.Applicable to numerous career fieldsDemonstrating fundamental principles, the concepts explored through these experiments are relevant to a host of career opportunities, including those in the health sciences, the nuclear power industry, regulatory agencies, and waste management services.

Experiments in Nuclear Science is an introductory-level laboratory manual providing hands-on opportunities for developing insights into the origins and properties of nuclear radiations, their interactions with matter, their detection and measurement, and their applications in the physical and life sciences. Based on experiments successfully performed by hundreds of students at Rutgers University and the University of Wisconsin, this manual can be used as a stand-alone volume or alongside a textbook such as Introduction to Nuclear Science by Jeff C. Bryan.Relevant to a range of coursesEach of the 32 exercises includes an overview of the scientific phenomenon, instructions for conducting the experiments and recording the data, directions for analyzing the data and reporting the results, specific questions relating to the experiments, and several problems relating to the scientific phenomena being investigated. Validated for safety and pedagogy in the undergraduate instructional laboratory, the exercises can be used in an undergraduate course in nuclear science. Individual exercises can also be adopted to demonstrate fundamental principles in a general science course as well as introductory biology and chemistry courses. Making use of off-the-shelf instrumentation, these exercises can be performed in a conventional laboratory under the supervision of an experienced instructor.Applicable to numerous career fieldsDemonstrating fundamental principles, the concepts explored through these experiments are relevant to a host of career opportunities, including those in the health sciences, the nuclear power industry, regulatory agencies, and waste management services.

The lungs provide a significant opportunity for the introduction of both therapeutic and toxic chemicals into the human body. In occupational and domestic environments, hazardous chemicals can enter the body through the lungs via gases, aerosols, and particulates from natural and anthropogenic sources. Fully updated with new research and discoveries since the last edition, Inhalation Toxicology, Third Edition presents contributions from internationally recognized scientists in the academic, commercial/industrial, and governmental sectors. A pragmatic resource for practicing professionals and students, the book comprehensively examines the relationship between the respiratory system and the toxicology of inhaled substances. Topics include: Regulatory aspects of exposure and testing Testing equipment and procedures Respiratory allergy and irritation of the respiratory tract Risk assessment Toxicology theory Toxicology modeling Toxic effects of some individual toxicantsNew topics in this third edition include collection and characterization of airborne particulate matter, the inhalation toxicology of asbestos fibers and nanoparticles, and the development of lung-on-a-chip technology for predicting in vivo responses. Each chapter concludes with thought-provoking questions and answers, enhancing the book’s educational utility.

Toxicity Assessment Alternatives: Methods, Issues, Opportunities contains a broad array of critical surveys, contributed by active and respected investigators, describing their research and offering updates on toxicity assessment alternatives, directions determined by current and future grant programs, opportunities for mechanistically based test methods to detect endocrine disruptor activity, the use of alternatives in the Department of Defense hazard assessment initiatives, and the issues and opportunities for validation and regulatory acceptance. Several of these advances make use oftransgenic models that reduce the time and cost of carcinogenicity testing. Others use tissue cultures for the assessment of endocrine disrupting chemicals. Cultures of human epidermal keratinocytes are applicable as models for sulfur mustard lesions, and in vitro protein denaturation is used as a chemical test for assessing the ocular and dermal irritation potential of cosmetic prod­ ucts. Molecular modeling is applied to explaining chemical toxicity. Commercially developed assay systems have undergone extensive evaluation by their manufacturers. Some of these await external valida­ tion, and others await acceptance by North American and European regulatory agencies. Toxicity Assessment Alternatives: Methods, Issues, Opportunities provides information from members of the scientific and regulatory communities on what has been achieved and what has been accepted in alternatives to animal testing.

Presents up-to-date concepts and approaches to the theory and practice of alternatives to animal testing and promotes technology transfer. The text addresses some of the ramifications of the National Institutes of Health Revitalization Act of 1993 which instructs the NIH to fund replacement, reduction and refinement alternatives. It also describes the likely impact of the European Union's Cosmetic Directive, which bans marketing of animal tested cosmetics in Europe after 1997 if alternatives are available. Contributions are grouped into five categories including: dermal toxicity; immunotoxicity; neurotoxicity; and oral/dermal/ocular validation.