Steven R Head – författare

After the death of his father, graduate student Wilson Dodge returns to his hometown in Wyoming to run the family newspaper. Its the mid-1950s: a time of simplicity and peace in Dodges small town until the newspapers youngest employee, photographer Corky Freeman, is found dead. For Dodge, nothing is simple anymore.Grayson Farmer is the Fremont County Sheriff , now facing reelection as a Democrat in the year of Eisenhower and the Republicans. He cant seem to find a suspect or motive in the death of Freeman, and the towns fresh-faced news editor does little but get in his way. Farmers problems only increase due to personnel problems, another death, and the FBI.Dodge sets out to solve Freemans murder and finds clues, romance, and family secrets. A journey that leads the newspaperman to lessons and dangers he had not expected. And as the body count rises and fills the front page, Dodge declares a deadline on reporting deaths.

This volume covers a wide range of various fields of research, with the common thread being Next Generation Sequencing (NGS) related methods and applications, as well as analysis and interpretation of the data obtained. Chapters guide readers through the highly dynamic processes of translational and transcriptional profiling of a cell, method to detect copy number alterations (CNAs), targeted sequencing applications,  method called “Hi-Plex” to characterize known polymorphic loci, single-cell of DNA or RNA, identify and characterize rare circulating CD4 T cells, and computational pipeline for RNAseq analysis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Next Generation Sequencing: Methodsand Protocols aims to be useful and informative for further study into this vital field.