Valdur A. Saks – författare

This text describes the use of quantitative methods in studies in organized metabolic systems, such as mathematical modelling and metabolic control analysis, for investigation of the problems of bioenergetics of the cell. It also presents experimental results. The following central problems of cellular bioenergetics are the focus of the discussions: the mechanisms of regulation of oxidative phosphorylation in cells "in vivo" in health and disease, the influence of fine intracellular structural organization on mitochondrial function, the intracellular networks of energy transfer and metabolic feedback signalling, the role of mitochondria in intracellular calcium cycling, and changes in mitochondrial membrane systems and cellular energetics under pathological conditions. Systematic studies from many laboratories presented in this volume show that many mitochondrial properties, including affinities for mitochondrial creatine kinase and its molecular biology are related to its molecular physiology and functional coupling to adenine nucleotide translocase, as well as its participation, together with the adenylate kinase system, in intracellular energy transfer.The results of the studies of creatine kinase-deficient transgenic mice are summarized and analyzed using mathematical models of the compartmentalized energy transfer, thus combining two research methods. All these results, together with physiological and NMR data on the cardiac metabolic and mitochondrial responses to workload changes, confirm the concept of metabolic networks of energy transfer and feedback regulation. The papers, mostly review articles, are written by experts in the field who analyze their experience of research and give insights into the future development of this increasingly important area of biological science.

Most of the information on the functional role, structure and molecular biology of creatine kinase is scattered in several hundreds of publications. The editors of this volume invited all authors who are most active at present in experimental research on creatine kinases to summarize their work and make the information easily available to a wider scientific audience, especially to young investigators who are entering this field. Since the functional role of coupled creatine kinases is directly related to the phenomenon of compartmentation and structural organization of metabolic networks, we also invited experts in the related areas to contribute to the volume. Almost all invited authors responded enthusiastically and the results of this collective work are presented here. For biochemists, biophysicists, physiologists, and medical research workers.

This volume continues the discussion of the problems of in vivo and in vitro. The recently solved X-ray structure of the mitochondrial creatine kinase and its molecular biology cellular bioenergetics - the tradition we started in 1994 by publication of the focused issue of Molecular and Cellular are analyzed with respect to its molecular physiology and Biochemistry, volume 133/134 and a book 'Cellular Bio­ functional coupling to the adenine nucleotide translocase, as energetics: role of coupled creatine kinases' edited by V. Saks well as its participation, together with the adenylate kinase and R. Ventura-Clapier and published by Kluwer Publishers, system, in intracellular energy transfer. The results of the Dordrecht -Boston. In the present volume, use of quantitative studies of creatine kinase deficient transgenic mice are methods of studies of organized metabolic systems, such as summarized and analyzed by using mathematical models of mathematical modeling and Metabolic Control Analysis, for the compartmentalized energy transfer, thus combining two investigation of the problems of bioenergetics of the cell is powerful new methods of the research. All these results, described together with presentation of new experimental together with the physiological and NMR data on the cardiac results. The following central problems of the cellular bio­ metabolic and mitochondrial responses to work-load changes energetics are the focus of the discussions: the mechanisms concord to the concept of metabolic networks of energy of regulation of oxidative phosphorylation in the cells in vivo transfer and feedback regulation.

Most of the information on the functional role, structure and molecular biology of creatine kinase is scattered in several hundreds of publications. The editors of this volume invited all authors who are most active at present in experimental research on creatine kinases to summarize their work and make the information easily available to a wider scientific audience, especially to young investigators who are entering this field. Since the functional role of coupled creatine kinases is directly related to the phenomenon of compartmentation and structural organization of metabolic networks, we also invited experts in the related areas to contribute to the volume. Almost all invited authors responded enthusiastically and the results of this collective work are presented here. For biochemists, biophysicists, physiologists, and medical research workers.