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This fourth edition provides all new methods on yeast that have not been included in the past three editions. Divided in four parts, chapters guide readers through general applications for genetic manipulation in yeast cells, genome-wide studies, systematically rearrange the yeast chromosomes, and pathway methods. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Yeast Protocol, Fourth Edition aims to compliment previous editions while offering new tools for the yeast research community.
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Yeast Protocols, Third Edition presents up-to-date advances in research using yeasts as models. Chapters cover topics such as basic protocols in yeast culture and genomic manipulation, protocols that study certain organelles such as mitochondria and peroxisomes and their functions in autophagy and assays commonly used in yeast-based studies that can be adapted to other organisms. As the first sequenced living organism, budding yeast S. cerevisiae and other model yeasts have helped greatly in life science research. The easy switch between the haploid and diploid state makes yeast a paradigm of genetic manipulation. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols and notes on troubleshooting and avoiding known pitfalls.
Authoritative and easily accessible, Yeast Protocols, Third Edition seeks to serve both professionals and novices with newly-developed protocols to study this essential model organism.
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Die Neuerscheinung behandelt die Entwicklung von Jherings Rechtsdenken, sie untersucht insbesondere die abstrakte Methodik in der konkreten Rechtsdogmatik. Vor dem Hintergrund der intensiven Diskussion um Kontinuität oder Diskontinuität im methodischen Denken Rudolf von Jherings unternimmt die Studie den Versuch, dieses juristische Methodendenken anhand verschiedener dogmatischer Beispiele genauer zu betrachten. Es wird gezeigt, dass sich eine starke methodische Kontinuität durch seine gesamte Rechtsdogmatik zieht. Der Autor arbeitet auf dem Gebiet der Jhering-Forschung, der neueren Privatrechtsgeschichte und der vergleichenden Rechtsgeschichte.
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