William M. Brown – författare

Knowledge of transcription has moved forward at a furious pace over recent years, and an understanding of the processes involved in gene regulation and expression has become an essential element in biochemistry, genome biology, molecular biology and molecular genetics. In this timely book, the authors present an accessible, yet comprehensive, coverage suitable for students at a senior undergraduate level, and for postgraduates needing an overview of the current state of play. It covers a number of pertinent examples of transcription systems for eukaryotes and prokaryotes, indicates methods for studying transcription, and surveys the whole topic of transcription from many perspectives.

Knowledge of transcription has moved forward at a furious pace over recent years, and an understanding of the processes involved in gene regulation and expression has become an essential element in biochemistry, genome biology, molecular biology and molecular genetics. In this timely book, the authors present an accessible, yet comprehensive, cover

Knowledge of transcription has moved forward at a furious pace over recent years, and an understanding of the processes involved in gene regulation and expression has become an essential element in biochemistry, genome biology, molecular biology and molecular genetics. In this timely book, the authors present an accessible, yet comprehensive, cover

DISCOVERY AND IDENTIFICATION OF FETUIN etuin was discovered in 1944 because of its behavior in the ultra- F 1 centrifuge. In his historical review of fetuin, Kai Pedersen, the original discoverer of the protein, wrote, "it (fetuin) was quite different from other serum proteins previously studied. It precipitated abundantly in an ammonium sulphate concentration range in which earlier only 7s and 20s components had been observed. Its molecular weight, 50,000, was the lowest observed for a serum protein; it was very asym- metrical; its partial specific volume was quite low-0. 70; the nitrogen content was low, 13g N/100g protein; the isoelectric point was low, pH 3. 5. The cause of the low nitrogen value was the high carbohydrate content of fetuin, 23%. The name fetuin (Latin; fetus) was proposed because this protein was the predominating component in the fetal serum, rapidly decreased, and finally disappeared in the newborn calf. " Proteins with fetuin-like properties were also identified in horse and sheep fetuses, and a small amount of a similar protein was identi- fied in human umbilical cord blood and fetal rabbit blood.' In the early 1950s, with improved fractionation methods, many 2 new proteins were isolated and described. In 1954 Harold Deutsch prepared a new purified fraction of fetuin that was more uniform than Pedersen' s. During the same period, another fetoprotein, a-fetopro- 3 fetal blood. Like fetuin, this tein (AFP), was first described in human new component was also an a -globulin.