Yohei Yamauchi – författare

This fully updated volume presents protocols crucial for contemporary influenza virus research, placing a special emphasis on the latest advancements in imaging technologies, including super-resolution microscopy, cryo-electron tomography (cryo-ET), freeze replica electron microscopy, and high-speed atomic force microscopy (AFM). In addition to covering traditional methods like virus purification, this edition introduces innovative approaches for visualizing molecular interactions between viral proteins and host proteins, methods for developing physiological cell culture models and animal models, as well as concepts concerning the prediction of viral evolution and the utilization of artificial intelligence in infection biology research. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and up-to-date, Influenza Virus: Methods and Protocols, Second Edition serves as an ideal guide for researchers looking to deepen our understanding of the intricate interplay between viruses and host cells.

This book provides researchers with widely used techniques for the study of virology, focusing on molecular biology and imaging to encourage mechanistic investigation of virus-host interactions. Chapters detail a broad range of methods from diagnosis, virus propagation, proteomics, haploid screening, lentiviral screening, virus entry, single molecule RNA imaging, correlative light and electron microscopy (CLEM), EM, light-sheet microscopy, biochemistry, viral transcription, physiological infection models, animal models, in vivo imaging, antigenic evolution, immunology to mathematical modelling. Reviews cover general influenza, clinical trials, both sides of the gain-of-function debate, and computational modelling. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and thorough, Influenza Virus: Methods and Protocols aims to motivate experienced researchers and newcomers in the field and improve our overall understanding of influenza.