Yutaka Shimada – författare

This volume focuses on the clinical applications of molecular diagnosis and targeted therapy from the viewpoint of oncologists specializing in specific organs. In addition, it discusses the role of molecular diagnosis and targeted therapy in the course of surgical treatment.Recent, rapid advances in molecular biology have shed new light on the mechanisms of cancer progression, and molecularly targeted drugs have been used to treat a variety of malignant diseases. For the diagnosis and precise treatment of cancer, analysis of the molecular background of the tumor is indispensable. However, there are both tumor-specific and comprehensive mechanisms involved in these processes. Accordingly, a firm grasp of molecular diagnosis and targeted therapies for different tumors is vital for clinicians and basic researchers alike. This book provides essential information and the latest findings on molecular diagnosis and targeted therapy for thoracic and gastrointestinal malignancies. Though primarily intended for clinical and basic oncologists, it also offers a useful guide for clinicians who are interested in this field and are considering getting started in molecular diagnosis and targeted therapy.

This volume focuses on the clinical applications of molecular diagnosis and targeted therapy from the viewpoint of oncologists specializing in specific organs. In addition, it discusses the role of molecular diagnosis and targeted therapy in the course of surgical treatment.Recent, rapid advances in molecular biology have shed new light on the mechanisms of cancer progression, and molecularly targeted drugs have been used to treat a variety of malignant diseases. For the diagnosis and precise treatment of cancer, analysis of the molecular background of the tumor is indispensable. However, there are both tumor-specific and comprehensive mechanisms involved in these processes. Accordingly, a firm grasp of molecular diagnosis and targeted therapies for different tumors is vital for clinicians and basic researchers alike. This book provides essential information and the latest findings on molecular diagnosis and targeted therapy for thoracic and gastrointestinal malignancies. Though primarily intended for clinical and basic oncologists, it also offers a useful guide for clinicians who are interested in this field and are considering getting started in molecular diagnosis and targeted therapy.

This book explores recent advances in the application of cell culture for experimental and clinical use. It addresses fundamental issues such as cell line misidentification and offers practical tips for its prevention. It also highlights the latest trends in human cell research for establishing disease models, drug discovery, and new therapeutic trials.The chapters provide a comprehensive guide to the establishment, maintenance, and passage methods of established cell lines, based on the history of cell line establishment. The book also details research activities and effective examples of clinical use of these established cell lines. In particular, dental pulp-derived stem cells are utilized as an example of primary culture, highlighting important perspectives in establishing clinical-grade standards for regenerative therapy and personalized medicine. It also covers innovative culture techniques of respiratory epithelial cells and their application to clinical studies. The book addresses the issue of cell line misidentification and clarifies the methods for determining the correct cell lines. Recent efforts to isolate and culture cells from trace amounts of clinical samples are also discussed, offering technical insights and outlining challenges for future applications of circulating tumor cells. Furthermore, it provides an overview of organoid culture and its applications in cancer research. The latter part of the book focuses on limitations of long-established cell lines suffering from genomic drift, a lack of clinical annotation, and the underrepresentation of rare cancers. Also, it describes the critical role of cancer cell lines in advancing rare-cancer research. The final chapter introduces research examples using iPS cells and AI in the field of neurodegenerative diseases, focusing on reprogramming technology.This book is intended for researchers, clinicians and students in the fields of cancer research, cell physiology, drug discovery and stem/iPS cell research.