Yves Durocher - Böcker
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Protein expression is an increasingly important tool for research on gene function. What is needed is not just a lab manual providing established methods as well as the latest state-of-the-art protocols, but also clear advice on what expression system to choose when. Expression Systems: Methods Express uniquely fills this need. It covers expression across a broad range of systems, including the following:Baculovirus expression vectorsCHO cellsE. coliHEK293-EBNA1 cellsLactococcus lactis and other gram positive bacteriaS. cerevisiaeTransfected insect cellsPichia pastorisMammalian cells using BacMam virusesLentiviral vectorsWheat germ cell-free systemThe book takes the reader through how to make an informed choice of appropriate system, taking into account the protein target, the time involved, the ultimate use of the expressed protein, and the laboratory equipment required. It also provides step-by-step methods for each system. In addition, the book describes the optimisation of expression strategies, expression engineering using ribosome display, and how to select protein variants with improved expression.Every chapter discusses the merits and limitations of the approaches available, describes the key techniques in full practical detail, and provides sensible advice for immediate use at the bench.In summary, Expression Systems: Methods Express is a comprehensive laboratory manual and information resource for researchers at all levels, from postgraduate student to principal investigator.
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Protein expression is an increasingly important tool for research on gene function. What is needed is not just a lab manual providing established methods as well as the latest state-of-the-art protocols, but also clear advice on what expression system to choose when. Expression Systems: Methods Express uniquely fills this need. It covers expression across a broad range of systems, including the following. *Baculovirus expression vectors*CHO cells* E. coli*HEK293-EBNA1 cells* Lactococcus lactis and other gram positive bacteria* S. cerevisiae*transfected insect cells* Pichia pastoris*mammalian cells using BacMam viruses*lentiviral vectors*wheat germ cell-free systemThe book takes the reader through how to make an informed choice of appropriate system, taking into account the protein target, the time involved, the ultimate use of the expressed protein, and the laboratory equipment required. It also provides step-by-step methods for each system. In addition, the book describes the optimisation of expression strategies, expression engineering using ribosome display, and how to select protein variants with improved expression. Every chapter discusses the merits and limitations of the approaches available, describes the key techniques in full practical detail, and provides sensible advice for immediate use at the bench. In summary, Expression Systems: Methods Express is a comprehensive laboratory manual and information resource for researchers at all levels, from postgraduate student to principal investigator.