David Wild is a healthcare industry veteran, with experience in biotechnology, pharmaceuticals, medical devices and immunodiagnostics, which remains his passion. He worked for Amersham, Eastman-Kodak, Johnson & Johnson, and Bristol-Myers Squibb, and consulted for diagnostics and biotechnology companies. He led research and development programs, design and construction of chemical and biotechnology plants, and integration of acquired companies. Director-level positions included Research and Development, Design Engineering, Operations and Strategy, for billion dollar businesses. He retired from full-time work in 2012 to focus on his role as Editor of The Immunoassay Handbook, and advises on product development, manufacturing and marketing.

"The beauty of this book is that it covers the whole field of immunoassay from reagents to method development, interferences and applications…The fourth edition does not disappoint. It is an essential reference manual for staff using immunoassays and to students in the field of laboratory medicine and other science disciplines." --Annals of Clinical Biochemistry, November 2013"The book starts out very basic with principles of immunoassays (‘immunoassays for beginners’) and builds progressively to discuss immunoassay components, configurations, development, implementation, product technology, applications other than clinical chemistry, and concludes with 23 chapters on clinical applications to human disease…This is the single ‘go-to’ book for any questions regarding immunoassays. If this is an area of interest for you, you must have this edition." --Doody.com, November 2013"Editors Wild, John, Sheehan, Binder, and He present this handbook on the highly sensitive technique of immunoassay…Equations, figures, and real-world examples are used to clarify mechanisms and analysis. The range of detail makes it appropriate as both a pedagogical resource and a reference for immunology labs." --Reference and Research Book News, October 2013"...it must be stated that there is much of value in the volume [2nd Ed.]. It is a useful and informative addition to any scientific library." --Robert F. Ritchie, Foundation for Blood Research, Scarborough, USA

• Part 1 IMMUNOASSAY FUNDAMENTALS1.1 How to use this book1.2 Immunoassays for beginners 1.3 Immunoassay performance measuresPart 2 IMMUNOASSAY CONFIGURATIONS2.1 Competitive and immunometric assays (including ELISA)2.2 Non-competitive immunoassays for small molecules2.3 Homogenous immunoassays2.4 Lateral flow immunoassay devices2.5 Ambient analyte immunoassay2.6 Free analyte immunoassay2.7 Qualitative immunoassays2.8 Immunoassays for the detection or quantification of antibodies2.9 Microsphere-based Multiplex Immunoassays: Development and Applications using Luminex xMAP Technology2.10 Lab on a chip2.11 Immunological Biosensors2.12 SPR2.13 Single Molecule ArrayPart 3 IMMUNOASSAY COMPONENTS3.1 Antibodies3.2 Signal generation systems3.3 Solid-phase and other separation systems3.4 Conjugation methods3.5 Standardisation and calibration3.6 Calibration curve-fittingPart 4 RELATED TECHNIQUES4.1 Foundations of immunochemistry4.2 Immunohistochemistry and immunofluorescencePart 5 IMMUNOASSAY DEVELOPMENT5.1 Practical guide to ELISA development5.2 Performance evaluation and validation5.3 Interferences in immunoassay5.4 Immunoassay development in industryPart 6 IMMUNOASSAY IMPLEMENTATION6.1 Subject preparation, sample collection and handling6.2 Laboratory quality assurance6.3 Point-of-care testing6.4 Choosing an automated immunoassay system6.5 Troubleshooting guidePart 7 IMMUNOASSAY PRODUCT TECHNOLOGY7.1 Introduction to product technology7.2 Market trends7.3 Lateral Flow and Consumer Diagnostics 7.4 Clearblue7.5 TRIAGE7.6 Stratus CS7.7 IMx7.8 AxSYM7.9 Architect7.10 Advia CENTAUR7.11 IMMULITE and IMMULITE 10007.12 IMMULITE 2000 and IMMULITE 2000 Xpi7.13 Eci7.14 Vitros Clin chem7.15 Access 27.16 Dxl 600 and 8007.17 Elecsys7.18 PRISM7.19 Phadia systemsPart 8 IMMUNOASSAY APPLICATIONS OTHER THAN CLINICAL CHEMISTRY8.1 Immunoassay applications in veterinary diagnostics8.2 Immunoassays in the pharmaceutical industryPart 9 IMMUNOASSAY CLINICAL APPLICATIONS (HUMAN)9.1 Clinical concepts9.2 Thyroid9.3 The adrenal cortex9.4 Bone metabolism9.5 Infertility9.6 In vitro fertilization and embryo transfer9.7 Hirsutism and virilization in the female9.8 Pregnancy9.9 Growth and growth hormone deficiency9.10 Diabetes mellitus9.11 Hematology9.12 Cardiac markers9.13 Cancer markers9.14 Allergy9.15 Autoimmune diseases9.16 GI Tract9.17 Hepatitis9.18 HIV9.19 Other viral diseases (excl HIV and Hepatitis)9.20 Other bacterial diseases9.21 Fungal and parasitic diseases9.22 Therapeutic drug monitoring9.23 Drugs of abuse

David Wild is a healthcare industry veteran, with experience in biotechnology, pharmaceuticals, medical devices and immunodiagnostics, which remains his passion. He worked for Amersham, Eastman-Kodak, Johnson & Johnson, and Bristol-Myers Squibb, and consulted for diagnostics and biotechnology companies. He led research and development programs, design and construction of chemical and biotechnology plants, and integration of acquired companies. Director-level positions included Research and Development, Design Engineering, Operations and Strategy, for billion dollar businesses. He retired from full-time work in 2012 to focus on his role as Editor of The Immunoassay Handbook, and advises on product development, manufacturing and marketing.

"The beauty of this book is that it covers the whole field of immunoassay from reagents to method development, interferences and applications…The fourth edition does not disappoint. It is an essential reference manual for staff using immunoassays and to students in the field of laboratory medicine and other science disciplines." --Annals of Clinical Biochemistry, November 2013"The book starts out very basic with principles of immunoassays (‘immunoassays for beginners’) and builds progressively to discuss immunoassay components, configurations, development, implementation, product technology, applications other than clinical chemistry, and concludes with 23 chapters on clinical applications to human disease…This is the single ‘go-to’ book for any questions regarding immunoassays. If this is an area of interest for you, you must have this edition." --Doody.com, November 2013"Editors Wild, John, Sheehan, Binder, and He present this handbook on the highly sensitive technique of immunoassay…Equations, figures, and real-world examples are used to clarify mechanisms and analysis. The range of detail makes it appropriate as both a pedagogical resource and a reference for immunology labs." --Reference and Research Book News, October 2013"...it must be stated that there is much of value in the volume [2nd Ed.]. It is a useful and informative addition to any scientific library." --Robert F. Ritchie, Foundation for Blood Research, Scarborough, USA

• Part 1 IMMUNOASSAY FUNDAMENTALS1.1 How to use this book1.2 Immunoassays for beginners 1.3 Immunoassay performance measuresPart 2 IMMUNOASSAY CONFIGURATIONS2.1 Competitive and immunometric assays (including ELISA)2.2 Non-competitive immunoassays for small molecules2.3 Homogenous immunoassays2.4 Lateral flow immunoassay devices2.5 Ambient analyte immunoassay2.6 Free analyte immunoassay2.7 Qualitative immunoassays2.8 Immunoassays for the detection or quantification of antibodies2.9 Microsphere-based Multiplex Immunoassays: Development and Applications using Luminex xMAP Technology2.10 Lab on a chip2.11 Immunological Biosensors2.12 SPR2.13 Single Molecule ArrayPart 3 IMMUNOASSAY COMPONENTS3.1 Antibodies3.2 Signal generation systems3.3 Solid-phase and other separation systems3.4 Conjugation methods3.5 Standardisation and calibration3.6 Calibration curve-fittingPart 4 RELATED TECHNIQUES4.1 Foundations of immunochemistry4.2 Immunohistochemistry and immunofluorescencePart 5 IMMUNOASSAY DEVELOPMENT5.1 Practical guide to ELISA development5.2 Performance evaluation and validation5.3 Interferences in immunoassay5.4 Immunoassay development in industryPart 6 IMMUNOASSAY IMPLEMENTATION6.1 Subject preparation, sample collection and handling6.2 Laboratory quality assurance6.3 Point-of-care testing6.4 Choosing an automated immunoassay system6.5 Troubleshooting guidePart 7 IMMUNOASSAY PRODUCT TECHNOLOGY7.1 Introduction to product technology7.2 Market trends7.3 Lateral Flow and Consumer Diagnostics 7.4 Clearblue7.5 TRIAGE7.6 Stratus CS7.7 IMx7.8 AxSYM7.9 Architect7.10 Advia CENTAUR7.11 IMMULITE and IMMULITE 10007.12 IMMULITE 2000 and IMMULITE 2000 Xpi7.13 Eci7.14 Vitros Clin chem7.15 Access 27.16 Dxl 600 and 8007.17 Elecsys7.18 PRISM7.19 Phadia systemsPart 8 IMMUNOASSAY APPLICATIONS OTHER THAN CLINICAL CHEMISTRY8.1 Immunoassay applications in veterinary diagnostics8.2 Immunoassays in the pharmaceutical industryPart 9 IMMUNOASSAY CLINICAL APPLICATIONS (HUMAN)9.1 Clinical concepts9.2 Thyroid9.3 The adrenal cortex9.4 Bone metabolism9.5 Infertility9.6 In vitro fertilization and embryo transfer9.7 Hirsutism and virilization in the female9.8 Pregnancy9.9 Growth and growth hormone deficiency9.10 Diabetes mellitus9.11 Hematology9.12 Cardiac markers9.13 Cancer markers9.14 Allergy9.15 Autoimmune diseases9.16 GI Tract9.17 Hepatitis9.18 HIV9.19 Other viral diseases (excl HIV and Hepatitis)9.20 Other bacterial diseases9.21 Fungal and parasitic diseases9.22 Therapeutic drug monitoring9.23 Drugs of abuse

David Wild is a healthcare industry veteran, with experience in biotechnology, pharmaceuticals, medical devices and immunodiagnostics, which remains his passion. He worked for Amersham, Eastman-Kodak, Johnson & Johnson, and Bristol-Myers Squibb, and consulted for diagnostics and biotechnology companies. He led research and development programs, design and construction of chemical and biotechnology plants, and integration of acquired companies. Director-level positions included Research and Development, Design Engineering, Operations and Strategy, for billion dollar businesses. He retired from full-time work in 2012 to focus on his role as Editor of The Immunoassay Handbook, and advises on product development, manufacturing and marketing.

"The beauty of this book is that it covers the whole field of immunoassay from reagents to method development, interferences and applications…The fourth edition does not disappoint. It is an essential reference manual for staff using immunoassays and to students in the field of laboratory medicine and other science disciplines." --Annals of Clinical Biochemistry, November 2013"The book starts out very basic with principles of immunoassays (‘immunoassays for beginners’) and builds progressively to discuss immunoassay components, configurations, development, implementation, product technology, applications other than clinical chemistry, and concludes with 23 chapters on clinical applications to human disease…This is the single ‘go-to’ book for any questions regarding immunoassays. If this is an area of interest for you, you must have this edition." --Doody.com, November 2013"Editors Wild, John, Sheehan, Binder, and He present this handbook on the highly sensitive technique of immunoassay…Equations, figures, and real-world examples are used to clarify mechanisms and analysis. The range of detail makes it appropriate as both a pedagogical resource and a reference for immunology labs." --Reference and Research Book News, October 2013"...it must be stated that there is much of value in the volume [2nd Ed.]. It is a useful and informative addition to any scientific library." --Robert F. Ritchie, Foundation for Blood Research, Scarborough, USA

• Part 1 IMMUNOASSAY FUNDAMENTALS1.1 How to use this book1.2 Immunoassays for beginners 1.3 Immunoassay performance measuresPart 2 IMMUNOASSAY CONFIGURATIONS2.1 Competitive and immunometric assays (including ELISA)2.2 Non-competitive immunoassays for small molecules2.3 Homogenous immunoassays2.4 Lateral flow immunoassay devices2.5 Ambient analyte immunoassay2.6 Free analyte immunoassay2.7 Qualitative immunoassays2.8 Immunoassays for the detection or quantification of antibodies2.9 Microsphere-based Multiplex Immunoassays: Development and Applications using Luminex xMAP Technology2.10 Lab on a chip2.11 Immunological Biosensors2.12 SPR2.13 Single Molecule ArrayPart 3 IMMUNOASSAY COMPONENTS3.1 Antibodies3.2 Signal generation systems3.3 Solid-phase and other separation systems3.4 Conjugation methods3.5 Standardisation and calibration3.6 Calibration curve-fittingPart 4 RELATED TECHNIQUES4.1 Foundations of immunochemistry4.2 Immunohistochemistry and immunofluorescencePart 5 IMMUNOASSAY DEVELOPMENT5.1 Practical guide to ELISA development5.2 Performance evaluation and validation5.3 Interferences in immunoassay5.4 Immunoassay development in industryPart 6 IMMUNOASSAY IMPLEMENTATION6.1 Subject preparation, sample collection and handling6.2 Laboratory quality assurance6.3 Point-of-care testing6.4 Choosing an automated immunoassay system6.5 Troubleshooting guidePart 7 IMMUNOASSAY PRODUCT TECHNOLOGY7.1 Introduction to product technology7.2 Market trends7.3 Lateral Flow and Consumer Diagnostics 7.4 Clearblue7.5 TRIAGE7.6 Stratus CS7.7 IMx7.8 AxSYM7.9 Architect7.10 Advia CENTAUR7.11 IMMULITE and IMMULITE 10007.12 IMMULITE 2000 and IMMULITE 2000 Xpi7.13 Eci7.14 Vitros Clin chem7.15 Access 27.16 Dxl 600 and 8007.17 Elecsys7.18 PRISM7.19 Phadia systemsPart 8 IMMUNOASSAY APPLICATIONS OTHER THAN CLINICAL CHEMISTRY8.1 Immunoassay applications in veterinary diagnostics8.2 Immunoassays in the pharmaceutical industryPart 9 IMMUNOASSAY CLINICAL APPLICATIONS (HUMAN)9.1 Clinical concepts9.2 Thyroid9.3 The adrenal cortex9.4 Bone metabolism9.5 Infertility9.6 In vitro fertilization and embryo transfer9.7 Hirsutism and virilization in the female9.8 Pregnancy9.9 Growth and growth hormone deficiency9.10 Diabetes mellitus9.11 Hematology9.12 Cardiac markers9.13 Cancer markers9.14 Allergy9.15 Autoimmune diseases9.16 GI Tract9.17 Hepatitis9.18 HIV9.19 Other viral diseases (excl HIV and Hepatitis)9.20 Other bacterial diseases9.21 Fungal and parasitic diseases9.22 Therapeutic drug monitoring9.23 Drugs of abuse