Membrane Protein Purification and Crystallization

A Practical Guide

Häftad, Engelska, 2003

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Beskrivning

This second edition of Membrane Protein Purification and Crystallization, A Practical Guide is written for bench scientists working in the fields of biochemistry, biology, and proteomic research. This guide presents isolation and crystallization techniques in a concise form, emphasizing the critical aspects unique to membrane proteins. It explains the principles of the methods and provides protocols of general use, permitting researchers and students new to this area to adapt these techniques to their particular needs. This edition is not only an update but is comprised mainly of new contributions. It is the first monograph compiling the essential approaches for membrane protein crystallization, and emphasizes recent progress in production and purification of recombinant membrane proteins.

Provides general guidelines and strategies for isolation and crystallization of membrane proteins
Gives detailed protocols that have wide application, and low specialized equipment needs
Emphasizes recent progress in production and purification of recombinant membrane proteins, especially of histidine-tagged and other affinity-epitope-tagged proteins
Summarizes recent developments of Blue-Native PAGE, a high resolution separation technique, which is independent of the use of recombinant techniques, and is especially suited for proteomic analyses of membrane protein complexes
Gives detailed protocols for membrane protein crystallization, and describes the production and use of antibody fragments for high resolution crystallization
Presents a comprehensive guide to 2D-crystallization of membrane proteins

Produktinformation

Utgivningsdatum:2003-01-05
Mått:191 x 235 x 15 mm
Vikt:590 g
Format:Häftad
Språk:Engelska
Antal sidor:316
Upplaga:2
Förlag:Elsevier Science
ISBN:9780123617767

Utforska kategorier

Biokemi inom Naturvetenskap och teknik
Cellbiologi inom Naturvetenskap och teknik
Biologi inom Naturvetenskap och teknik

Mer om författaren

Deceased: February 12, 2017

Innehållsförteckning

I. Strategies and TechniquesPurification Strategies for Membrane ProteinsChromatographic Techniques and Basic Operations in Membrane Protein PurificationProduction and purification of recombinant membrane proteinsSDS Electrophoresis TechniquesBlue-Native ElectrophoresisPreparative Isoelectric FocussingMembrane protein crystallizationII. Discussion of Selected Isolation ProtocolsLipid Dependent Inactivation and Reactivation of Bovine complex IIIPurification of affinity-epitope tagged G-protein coupled receptorsPurification of NhaA, the Na+/H+ Antiporter of Escherichia coli for 3D or 2DCrystallizationPurification of the yeast cytochrome bc1 complexIII. Crystallization of Membrane ProteinsAntibody fragment-mediated crystallization of membrane proteinsCrystallization of Wolinella succinogenes quinol:fumarate reductaseBa3-type cytochrome-c oxidase from Thermus thermophiluspurification, crystallization, and crystal transformationTwo-dimensional crystallization of membrane proteins: a practical guideIn cubo crystallization of membrane proteins