Globins and Other Nitric Oxide-Reactive Proteins, Part A

Professor Robert K Poole is Emeritus Professor of Microbiology at the University of Sheffield, UK. He was previously West Riding Professor of Microbiology at Sheffield and until 1996 held a Personal Chair in Microbiology at King’s College London. During his long career, he has been awarded several research Fellowships, and taken sabbatical leave at the Australian National University, Kyoto University and Cornell University. His career-long interests have been in the areas of bacterial respiratory metabolism, metal-microbe interactions and bioactive small gas molecules. In particular, he has made notable contributions to bacterial terminal oxidases and resistance to nitric oxide with implications for bacterial pathogenesis. He co-discovered the flavohaemoglobin Hmp, now recognised as the preeminent mechanism of nitric oxide resistance in bacteria. He has served as Chairman of numerous research council grant committees, held research grants for over 40 years and published extensively (h-index, 2024 = 70). He served on several Institute review panels in the UK and overseas. He is a Fellow of the Royal Society of Chemistry and the Royal Society of Biology.

• Section I. Nitric Oxide: Chemical and Analytical MethodsChapter 1The Chemistry of Nitric Oxide and Related SpeciesMartin H. HughesChapter 2Delivery of nitric oxide for analysis of the function of cytochrome c’.Lindsay J. Cole, Wilhelmina M. Huston, and James W.B. MoirChapter 3The preparation and purification of NO gas and the use of NO releasers. The application of NO donors and other agents of nitrosative stress in biological systems Rubina G. Aga and Martin N. HughesChapter 4The Chemistry of Peroxynitrite – Implications for Biological ActivitySara Goldstein and Gabor MerényiChapter 5Nitric Oxide (NO) Selective ElectrodesIan R. Davies, Xueji ZhangChapter 6NO, N2O and O2 Reaction Kinetics: Scope and Limitations of the Clark ElectrodeL.A.M. Pouvreau, M. J. F. Strampraad, S. Van Berloo, J. H. Kattenberg and S. de VriesChapter 7Chemiluminescence quantification of Nitric oxide and its derivatives in liquid samplesJay R. Laver, Tânia M. Stevanin, Robert C. ReadSection II. Bacterial and Archaeal HemoglobinsChapter 8Interactions of nitric oxide with hemoglobin: from microbes to manMichael Angelo, Alfred Hausladen and Jonathan S. StamlerChapter 9Expression and purification of E. coli HmpRobert K. Poole Chapter 10Structural studies on flavohemoglobinsAndrea Ilari and Alberto BoffiChapter 11Flavohemoglobin of Staphylococcus aureusLígia S. Nobre, Vera L. Gonçalves and Lígia M. SaraivaChapter 12Assay and Characterization of the Nitric Oxide Dioxygenase Activity of (Flavo)HemoglobinsPaul R. GardnerChapter 13Globin Interactions with Lipids and MembranesAntonio Di Giulio and Alessandra BonamoreChapter 14Assessment of Biotechnologically Relevant Characteristics of Heterologous Hemoglobins in Escherichia coliPauli T. Kallio, Christian J. T. Bollinger, Taija Koskenkorva, and Alexander D. FreyChapter 15Applications of the Vitreoscilla hemoglobin (VHb) gene (vgb) for improved microbial fermentation processesXiao-Xing WEI, Guo-Qiang CHENChapter 16Expression and Purification of Cgb and Ctb, the NO-Inducible Globins of the Foodborne Bacterial Pathogen Campylobacter jejuniJames L. Pickford, Laura Wainwright, Guanghui Wu, Robert K. PooleChapter 17Mapping Heme-Ligand Tunnel in Group I Truncated(2/2) Hemoglobins Alessandra Pesce, Mario Milani, Marco Nardini and Martino BolognesiChapter 18Scavenging of reactive nitrogen species by mycobacterial truncated hemoglobinsPaolo Ascenzi and Paolo ViscaSection III. Other HemoglobinsChapter 19Expression, purification and crystallisation of neuro-and cytoglobinSylvia Dewilde, Kirsten Mees, Laurent Coger, Christophe Lechauve, Michael C. Marden, Alexandra Pesce, Martino Bolognesi, Luc MoneesChapter 20Measurement of distal histidine coordination equilibrium and kinetics in hexacoordinate hemoglobins.Benoit J. Smagghe, Puspita Halder, and Mark S. HargroveChapter 21Purification of Class 1 Plant Hemoglobins and Examination of their Functional PropertiesAbir U. Igamberdiev and Robert D. HillChapter 22Use of in silico (computer) methods to predict and analyze the tertiary structure of plant hemoglobinsSabarinathan Kuttalingam Gopalasubramaniam, Verónica Garrocho-Villegas, Genoveva Bustos Rivera, Nina Pastor and Raúl Arredondo-PeterChapter 23A Self-induction Method to Produce High Quantities of Recombinant Functional Flavo-Leghemoglobin Reductase.Estibaliz Urarte, Iñigo Auzmendi, Selene Rol, Idoia Ariz, Pedro Aparicio-Tejo, Raúl Arredondo-Peter and Jose F. MoranChapter 24Spectroscopic and crystallographic characterization of bis-histidyl adducts in tetrameric hemoglobinsAlessandro Vergara, Luigi Vitagliano, Cinzia Verde, Guido di Prisco, Lelio MazzarellaChapter 25Dinitrosyl Iron Complexes Bound with Haemoglobin as Markers of Oxidative StressKonstantin B. Shumaev, Olga V. Kosmachevskaya, Alexandr A. Timoshin, Anatoly F. Vanin and Alexey F. TopunovChapter 26Linked analysis of large cooperative, allosteric systems: the case of the giant HBL hemoglobinsNadja Hellmann, Roy E. Weberb and Heinz DeckerChapter 27Mass Mapping of Large Globin Complexes by Scanning Transmission Electron MicroscopyJoseph S. Wall, Martha N. Simon, Beth Y. Lin, Serge N. VinogradovChapter 28Mini-Hemoglobins from Nemertean WormsThomas L. Vandergon and Austen F. RiggsChapter 29Comparative and Evolutionary Genomics of Globin Genes in FishEnrico Negrisolo, Luca Bargelloni, Tomaso Patarnello, Catherine Ozouf-Costaz, Eva Pisano, Guido di Prisco, Cinzia VerdeChapter 30Inferring Evolution of Fish Proteins: The Globin Case StudyAgnes Dettai, Guideo de Prisco, Guillaume Lecointre, Elio Parisi, Cinzia VerdeChapter 31Tracing Globin Phylogeny Using PSI-BLAST Searches Based on Groups of Sequences Serge N. Vinogradov