Daniel Lee Purich has been at the forefront of biochemistry research for more than 25 years. He was awarded the National Institutes of Health Research Career Development Award from 1977–1982, the Plous Teaching Award (the University of California Santa Barbara Campus-Wide Teaching Award) in 1977, has been a member of the National Institutes of Health Biochemistry Study Section from 1982–1985, and a member of the Journal of Biological Chemistry Editorial Board from 1981–1986. He has been a member of the American Society of Biological Chemists, the American Chemical Society, the New York Academy of Sciences, the Biochemical Society, and the American Society for Cell Biology. Dr. Purich is currently a Professor and Chairman of the Department of Biochemistry and Molecular Biology at the Florida College of Medicine. He is the author and editor of numerous scientific publications.

• Section 1: Initial Rate Theory & Methods1. Derivation of Initial Velocity and Isotope Exchange Rate Equation (Huang)2. Practical Considerations in the Design of Initial Velocity Enzyme Rate Assays (Purich)3. Techniques in Coupled Enzyme Rate Assays (Rudolph)4. Regression Analysis, Experimental Error, and Statistical Criteria in the Design and Analysis of Experiments for Discrimination between Rival Kinetic Models (Mannervik)5. Analysis of Enzyme Progress Curves by Nonlinear Regression (Duggleby)6. Effects of pH on Enzymes (Tipton)7. Temperature Effects on Enzyme Kinetics (Laidler)8. Site-Directed Mutagenesis: A Tool for Studying Enzyme Catalysis (Plapp)9. Cooperativity in Enzyme Function: Equilibrium and Kinetic Aspects (Neet)Section 2: Inhibitors as Probes of Enzyme Catalysis10. Reversible Enzyme Inhibitors as Mechanistic Probes (Fromm)11. Application of Affinity Labeling for Studying Enzyme Structure and Function (Plapp)12. Mechanism-Based Enzyme Inactivators (Silverman)Section 3: Detection of Enzyme Reaction Intermediates13. Transient Kinetic Approaches to Enzyme Mechanisms (Hammes)14. Rapid Quench Kinetic Analysis of Polymerases, Adenosinetriphosphatases, and Enzyme Intermediates (Johnson)15. Pre-Steady-State Kinetics of Enzymatic Reactions Studied by Electrospray Mass Spectrometry with Rapid On-Line Techniques (Konermann)Section 4. Isotopic Probes of Enyme Processes16. Isotope Exchange Methods for Elucidating Enzyme Catalysis (Purich)17. Positional Isotope Exchange as Probes of Enzyme Action (Raushel)18. Enzymatic Transition State Analysis and Transition State Analogues (Schramm)19. Determining Transition-States from Kinetic Isotope Effects (Berti)20. Computational Methods for Transition State and Inhibitor Recognition (Schwartz)

Daniel Lee Purich has been at the forefront of biochemistry research for more than 25 years. He was awarded the National Institutes of Health Research Career Development Award from 1977–1982, the Plous Teaching Award (the University of California Santa Barbara Campus-Wide Teaching Award) in 1977, has been a member of the National Institutes of Health Biochemistry Study Section from 1982–1985, and a member of the Journal of Biological Chemistry Editorial Board from 1981–1986. He has been a member of the American Society of Biological Chemists, the American Chemical Society, the New York Academy of Sciences, the Biochemical Society, and the American Society for Cell Biology. Dr. Purich is currently a Professor and Chairman of the Department of Biochemistry and Molecular Biology at the Florida College of Medicine. He is the author and editor of numerous scientific publications.

• Section 1: Initial Rate Theory & Methods1. Derivation of Initial Velocity and Isotope Exchange Rate Equation (Huang)2. Practical Considerations in the Design of Initial Velocity Enzyme Rate Assays (Purich)3. Techniques in Coupled Enzyme Rate Assays (Rudolph)4. Regression Analysis, Experimental Error, and Statistical Criteria in the Design and Analysis of Experiments for Discrimination between Rival Kinetic Models (Mannervik)5. Analysis of Enzyme Progress Curves by Nonlinear Regression (Duggleby)6. Effects of pH on Enzymes (Tipton)7. Temperature Effects on Enzyme Kinetics (Laidler)8. Site-Directed Mutagenesis: A Tool for Studying Enzyme Catalysis (Plapp)9. Cooperativity in Enzyme Function: Equilibrium and Kinetic Aspects (Neet)Section 2: Inhibitors as Probes of Enzyme Catalysis10. Reversible Enzyme Inhibitors as Mechanistic Probes (Fromm)11. Application of Affinity Labeling for Studying Enzyme Structure and Function (Plapp)12. Mechanism-Based Enzyme Inactivators (Silverman)Section 3: Detection of Enzyme Reaction Intermediates13. Transient Kinetic Approaches to Enzyme Mechanisms (Hammes)14. Rapid Quench Kinetic Analysis of Polymerases, Adenosinetriphosphatases, and Enzyme Intermediates (Johnson)15. Pre-Steady-State Kinetics of Enzymatic Reactions Studied by Electrospray Mass Spectrometry with Rapid On-Line Techniques (Konermann)Section 4. Isotopic Probes of Enyme Processes16. Isotope Exchange Methods for Elucidating Enzyme Catalysis (Purich)17. Positional Isotope Exchange as Probes of Enzyme Action (Raushel)18. Enzymatic Transition State Analysis and Transition State Analogues (Schramm)19. Determining Transition-States from Kinetic Isotope Effects (Berti)20. Computational Methods for Transition State and Inhibitor Recognition (Schwartz)

Daniel Lee Purich has been at the forefront of biochemistry research for more than 25 years. He was awarded the National Institutes of Health Research Career Development Award from 1977–1982, the Plous Teaching Award (the University of California Santa Barbara Campus-Wide Teaching Award) in 1977, has been a member of the National Institutes of Health Biochemistry Study Section from 1982–1985, and a member of the Journal of Biological Chemistry Editorial Board from 1981–1986. He has been a member of the American Society of Biological Chemists, the American Chemical Society, the New York Academy of Sciences, the Biochemical Society, and the American Society for Cell Biology. Dr. Purich is currently a Professor and Chairman of the Department of Biochemistry and Molecular Biology at the Florida College of Medicine. He is the author and editor of numerous scientific publications.

• Section 1: Initial Rate Theory & Methods1. Derivation of Initial Velocity and Isotope Exchange Rate Equation (Huang)2. Practical Considerations in the Design of Initial Velocity Enzyme Rate Assays (Purich)3. Techniques in Coupled Enzyme Rate Assays (Rudolph)4. Regression Analysis, Experimental Error, and Statistical Criteria in the Design and Analysis of Experiments for Discrimination between Rival Kinetic Models (Mannervik)5. Analysis of Enzyme Progress Curves by Nonlinear Regression (Duggleby)6. Effects of pH on Enzymes (Tipton)7. Temperature Effects on Enzyme Kinetics (Laidler)8. Site-Directed Mutagenesis: A Tool for Studying Enzyme Catalysis (Plapp)9. Cooperativity in Enzyme Function: Equilibrium and Kinetic Aspects (Neet)Section 2: Inhibitors as Probes of Enzyme Catalysis10. Reversible Enzyme Inhibitors as Mechanistic Probes (Fromm)11. Application of Affinity Labeling for Studying Enzyme Structure and Function (Plapp)12. Mechanism-Based Enzyme Inactivators (Silverman)Section 3: Detection of Enzyme Reaction Intermediates13. Transient Kinetic Approaches to Enzyme Mechanisms (Hammes)14. Rapid Quench Kinetic Analysis of Polymerases, Adenosinetriphosphatases, and Enzyme Intermediates (Johnson)15. Pre-Steady-State Kinetics of Enzymatic Reactions Studied by Electrospray Mass Spectrometry with Rapid On-Line Techniques (Konermann)Section 4. Isotopic Probes of Enyme Processes16. Isotope Exchange Methods for Elucidating Enzyme Catalysis (Purich)17. Positional Isotope Exchange as Probes of Enzyme Action (Raushel)18. Enzymatic Transition State Analysis and Transition State Analogues (Schramm)19. Determining Transition-States from Kinetic Isotope Effects (Berti)20. Computational Methods for Transition State and Inhibitor Recognition (Schwartz)