Nuclear Mechanics and Genome Regulation

Dr. G. V. Shivashankar is currently the Deputy Director of Mechanobiology Institute, National University of Singapore. Shivashankar’s laboratory is focused on understanding the role of cell geometry on nuclear mechanics and genome regulation in living cells using a multi-disciplinary approach. He carried out his PhD research at the Rockefeller University (1994-1999) and Postdoctoral research at NEC Research Institute, Princeton, USA (1999-2000). He started his laboratory at the National Center for Biological Sciences, TIFR- Bangalore, India (2000-2009) before relocating to a tenured faculty position at the National University of Singapore in 2009. His scientific awards include the Birla Science Prize (2006) and the Swarnajayanthi Fellowship (2007), and he was elected to the Indian Academy of Sciences (2010). He also edited a Methods in Cell Biology volume on Nuclear Mechanics and Genome Regulation (2010), published by Elsevier. More recently, he headed the Joint Research Laboratory with the FIRC Institute of Molecular Oncology (IFOM), Milan, Italy and was appointed as an IFOM-NUS Chair Professor in 2014.

• Section-A: Cell Nucleus: Organization and MechanoBiology Fluorescence Fluctuation Microscopy to reveal 3D-Architecture and Function in the Cell NucleusThorsten Lenser1,2, Klaus Weisshart2, Tobias Ulbricht3, Karolin Klement3, and Peter HemmerichStudying histone modifications and their genomic functions by employing chromatin immunoprecipitation and immunoblottingRanveer S. Jayani, Praveena L. Ramanujam and Sanjeev GalandeDynamic organization of chromatin assembly & transcription factories in living cellsBidisha Sinha, Dipanjan Bhattacharya, Deepak Kumar Sinha, Shefali Talwar, Shovamayee Maharana, Soumya Gupta and G. V.ShivashankarManipulation & Isolation of Single Cells & NucleiSwee Jin Tan, Qingsen Li and Chwee Teck LimBeyond lamins: other structural components of the nucleoskeletonZhixia Zhong , Katherine L. Wilson and Kris Noel DahlAltered mechanical properties of the nucleus in diseaseMaria Lucia Lombardi and Jan LammerdingTheoretical concepts and models of cellular mechanosensingRumi De, Assaf Zemel and Samuel A. SafranSection-B: Impact of Nuclear Mechanics on FunctionMechanical Induction of Gene Expression in Connective Tissue CellsMathew Chan, Boris Hinz, Christopher A. McCullochPhysical plasticity of the Nucleus and its manipulationIrena Ivanovska, Joe Swift, Takamasa Harada, J. David Pajerowski and Dennis E. DischerPrestressed Nuclear Organization in Living CellsAprotim Mazumder, Roopa T. , Abhishek Kumar, K.Venkatesan Iyer, Nisha M Ramdas and G. V.ShivashankarNanotopography/mechanical induction of stem-cell differentiationEvelyn YimMechanical Induction in Embryonic Development and Tumour Growth: Integrative Cues through Molecular to Multi-cellular Interplay, and Evolutionary PerspectivesMaria-Elena Fernandez-Sanchez, Fanny Serman, Padra Ahmadi and Emmanuel FargeInformatics based analysis of mechanosignaling in the laminopathiesFrank P.L. Lai, Radfidah A. Mutalif, Siew Cheng Phua, and Colin L. StewartAutosomal Dominant Leukodystrophy caused by lamin B1 duplications: A clinical and molecular case study of altered nuclear function and disease Quasar Saleem Padiath and Ying-Hui Fu