Cellulases

Inbunden, Engelska, 2012

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Beskrivning

Cellulase refers to a class of enzymes produced chiefly by fungi, bacteria, and protozoans that catalyze cellulolysis. This volume of Methods in Enzymology comprehensively covers this topic. With an international board of authors, this volume covers subjects such as "The DNSA reducing assay for measuring cellulases," "Measuring processivity" and "In situ cellulose detection with carbohydrate-binding modules."

Comprehensively covers the topic of cellulases
International board of authors

Produktinformation

Utgivningsdatum:2012-07-10
Mått:152 x 229 x undefined mm
Vikt:880 g
Format:Inbunden
Språk:Engelska
Serie:Methods in Enzymology
Antal sidor:552
Förlag:Elsevier Science
Medarbetare:Harry Gilbert
ISBN:9780124159310

Utforska kategorier

Biokemi inom Naturvetenskap och teknik

Innehållsförteckning

Measurement of endo-1,4-b-glucanaseBiomass Conversion determined via Fluorescent Cellulose Decay assayThe analysis of saccharification in biomass using an automated high-throughput methodStudies of enzymatic cleavage of cellulose using Polysaccharide Analysis by Carbohydrate gel Electrophoresis (PACE)Measuring processivityDistinguishing xyloglucanase activity in endo-b (1® 4) glucanasesMethods for structural characterization of the products of cellulose- and xyloglucan-hydrolyzing enzymesThe crystallization and structural analysis of cellulases (and other glycoside hydrolases): strategies and tacticsVisualization of Cellobiohydrolase I from Trichoderma reesei Moving on Crystalline Cellulose Using High-Speed Atomic Force MicroscopySmall angle X-ray scattering and crystallography; a winning combination for exploring the multi-modular organisation of cellulolytic macromolecular complexesQuantitative approaches to the analysis of carbohydrate-binding module functionIn Situ Detection Of Cellulose With Carbohydrate-Binding ModulesInteractions between family 3 carbohydrate binding modules (CBMs) and cellulosomal linker peptidesApproaches for improving thermostability characteristics in cellulasesThermophilic glycosynthases for oligosaccharides synthesisEngineering cellulase activity into Clostridium acetobutylicum Transformation of Clostridium thermocellum by electroporationGenetic and Functional Genomic Approaches for the Study of Plant Cell Wall Degradation in Cellvibrio japonicusMethods for the isolation of cellulose-degrading microorganismsMetagenomic approaches to the discovery of cellulasesEscherichia coli expression, purification, crystallization and structure determination of bacterial cohesin-dockerin complexesMeasurements of relative binding of cohesin and dockerin mutants using an advanced ELISA technique for high-affinity interactionsDesigner cellulosomes for enhanced hydrolysis of cellulosic substratesHigh-throughput screening of cohesin mutant libraries on cellulose microarrays