Membrane Proteins - Engineering, Purification and Crystallization

Dr. Arun K. Shukla is a world leader in the field of GPCR biology and he is currently a Professor in the Department of Biological Sciences and Bioengineering at the Institute of Technology, Kanpur in India. Dr. Shukla’s research program is focused on understanding the structure, function and regulation of G protein-coupled receptors with a long-term of designing novel therapeutics with minimized side-effects.

• Section I. Membrane Protein Engineering, Solubilization and Purification1. Multicolor fluorescence-based screening toward structural analysis of multiprotein membrane complexesSimon Trowitzsch  & Robert Tampé 2. A novel screening approach for optimal and functional fusion of T4 lysozyme in GPCRsElizabeth Mathew and Mark E Dumont3. Membrane preparation and solubilizationAnkita Roy 4. Amphipathic agents for membrane protein studyAiman Sadaf, Kyung Ho Cho, Bernadette Byrne, Pil Seok Chae 5. Quantification of detergent using colorimetric methods in membrane protein crystallographyChelsy Prince , Zongchao Jia6. Solubilization of G Protein-Coupled Receptors: A Convenient Strategy to Explore Lipid-Receptor InteractionAmitabha Chattopadhyay, Bhagyashree D. Rao and Md. Jafurulla7. Overexpression, Isolation, Purification and Crystallization of NhaAEtana Padan and Manish Dwivedi8. Purification, refolding and crystallization of the outer membrane protein OmpG from Escherichia coliStefan Köster, Katharina van Pee, Özkan Yildiz 9. Biophysical Approaches to the Study of LeuT, a Prokaryotic Homolog of Neurotransmitter Sodium  SymportersSatinder K. Singh and Aritra PalSection II. Generation and Use of Antibody Fragments Against Membrane Proteins10. Generation of recombinant antibody fragments for membrane protein crystallizationSyed H. Mir , Claudia Escher, Wei-Chun Kao, Dominic Birth, Christophe Wirth, Carola Hunte11. Phage Display Selections for Affinity Reagents to Membrane Proteins in NanodiscsPawel K. Dominik, and Anthony A. Kossiakoff12. Antibody fragments for crystallization, trapping of active conformations and stabilization of G-protein coupled receptors and their signaling complexesArun K. Shukla, Charu Gupta, Ashish Srivastava and Deepika JaimanSection III. Biophysical Studies of  Membrane Proteins13. Conformational Analysis of G Protein-Coupled Receptor Signaling by Hydrogen/Deuterium Exchange Mass SpectrometrySheng Li, Su Youn Lee and Ka Young Chung14. EPR Studies of Gating Mechanisms in Ion ChannelsSudha Chakrapani15. Magic-Angle-Spinning Solid-State NMR of Membrane ProteinsLindsay A Baker, Gert E Folkers, Tessa Sinnige, Klaartje Houben, Mohammed Kaplan, Elwin AJ van der Cruijsen, Marc Baldus16. Solution NMR Structure Determination of Polytopic α-helical membrane proteins: A guide to spin label paramagnetic relaxation enhancement restraintsLinda Columbus and Brett KronckeSection IV. Crystallization of Membrane Proteins17. Inducing Two-Dimensional Crystallization of Membrane Proteins by Dialysis for Electron CrystallographyYusuf M. Uddin and Ingeborg Schmidt-Krey18. Crystallization of Membrane Proteins by Vapor DiffusionJared A. Delmar, Jani Reddy Bolla, Chih-Chia Su, and Edward W. Yu19. An empirical approach to bicelle crystallizationSandra Poulos, Jacob L. W. Morgan, Jochen Zimmer, Salem Faham20. Fluorescence Recovery After Photobleaching in Lipidic Cubic Phase (LCP-FRAP): A pre-crystallization assay for membrane proteinsGustavo Fenalti, Enrique E. Abola, Chong Wang, Beili Wu, Vadim Cherezov21. Crystallization of Proteins from Crude Bovine Rod Outer SegmentsBo Y Baker, Sahil Gulati, Wuxian Shi, Benlian Wang, Phoebe L. Stewart, and Krzysztof Palczewski22. Crystallization of Photosystem II for Time Resolved Structural Studies Using an X-ray Free Electron LaserJesse Coe, Christopher Kupitz, Shibom Basu, Chelsie E. Conrad, Shatabdi Roy-Chowdhury, Raimund Fromme, Petra FrommeSection V. Computational Approaches to Understand Membrane Proteins23. Major Intrinsic Protein Superfamily: Channels with Unique Structural Features and Diverse Selectivity FiltersRavi Kumar Verma, Anjali Bansal Gupta and Ramasubbu Sankararamakrishnan24. Comparative Sequence-Function Analysis of the Major Facilitator Superfamily: the ‘Mix-and-Match’ MethodM. Gregor Madej25. Elucidating Ligand-Modulated Conformational Landscape of GPCRs Using Cloud-computing ApproachesDiwakar Shukla, Morgan Lawrenz and Vijay S. Pande